ArticleNeuron2020
Precision Calcium Imaging of Dense Neural Populations via a Cell-Body-Targeted Calcium Indicator.
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Article in Neuron, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 78 papers.
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78citing papers in PubMed
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Who cites it
78 citing papers in PubMed, 139 citations in OpenAlex.
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18 more citing papers are in PubMed but not listed here.
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Authors and funding
34 authors at 14 institutions in 4 countries.
Or A ShemeshThe MIT Media Laboratory, Massachusetts Institute of Technology (MIT), Cambridge, MA, USA; Department of Biological Engineering, MIT, Cambridge, MA, USA; MIT Center for Neurobiological Engineering, MIT, Cambridge, MA, USA; Department of Brain and Cognitive Sciences, MIT, Cambridge, MA, USA; MIT McGovern Institute for Brain Research, MIT, Cambridge, MA, USA; Department of Neurobiology and Pittsburgh Institute for Neurodegenerative Diseases, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
Changyang LinghuThe MIT Media Laboratory, Massachusetts Institute of Technology (MIT), Cambridge, MA, USA; MIT Center for Neurobiological Engineering, MIT, Cambridge, MA, USA; MIT McGovern Institute for Brain Research, MIT, Cambridge, MA, USA; Department of Electrical Engineering and Computer Science, MIT, Cambridge, MA, USA.
Kiryl D PiatkevichThe MIT Media Laboratory, Massachusetts Institute of Technology (MIT), Cambridge, MA, USA; Department of Biological Engineering, MIT, Cambridge, MA, USA; MIT Center for Neurobiological Engineering, MIT, Cambridge, MA, USA; Department of Brain and Cognitive Sciences, MIT, Cambridge, MA, USA; MIT McGovern Institute for Brain Research, MIT, Cambridge, MA, USA; School of Life Sciences, Westlake University, Hangzhou, Zhejiang Province, China.
Daniel GoodwinThe MIT Media Laboratory, Massachusetts Institute of Technology (MIT), Cambridge, MA, USA; MIT Center for Neurobiological Engineering, MIT, Cambridge, MA, USA; MIT McGovern Institute for Brain Research, MIT, Cambridge, MA, USA.
Orhan Tunc CelikerMIT McGovern Institute for Brain Research, MIT, Cambridge, MA, USA; Department of Electrical Engineering and Computer Science, MIT, Cambridge, MA, USA.
Howard J GrittonBoston University, Department of Biomedical Engineering, Boston, MA 02215, USA.
Michael F RomanoBoston University, Department of Biomedical Engineering, Boston, MA 02215, USA.
Ruixuan GaoThe MIT Media Laboratory, Massachusetts Institute of Technology (MIT), Cambridge, MA, USA; MIT McGovern Institute for Brain Research, MIT, Cambridge, MA, USA.
Chih-Chieh Jay YuThe MIT Media Laboratory, Massachusetts Institute of Technology (MIT), Cambridge, MA, USA; Department of Biological Engineering, MIT, Cambridge, MA, USA; MIT McGovern Institute for Brain Research, MIT, Cambridge, MA, USA.
Hua-An TsengBoston University, Department of Biomedical Engineering, Boston, MA 02215, USA.
Seth BensussenBoston University, Department of Biomedical Engineering, Boston, MA 02215, USA.
Sujatha NarayanJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
Chao-Tsung YangJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
Limor FreifeldThe MIT Media Laboratory, Massachusetts Institute of Technology (MIT), Cambridge, MA, USA; MIT Center for Neurobiological Engineering, MIT, Cambridge, MA, USA; MIT McGovern Institute for Brain Research, MIT, Cambridge, MA, USA; Faculty of Biomedical Engineering, Technion, Haifa, Israel.
Cody A SicilianoVanderbilt Center for Addiction Research, Department of Pharmacology, Vanderbilt University, Nashville, TN 37232, USA.
Ishan GuptaThe MIT Media Laboratory, Massachusetts Institute of Technology (MIT), Cambridge, MA, USA; Department of Biological Engineering, MIT, Cambridge, MA, USA; MIT Center for Neurobiological Engineering, MIT, Cambridge, MA, USA; MIT McGovern Institute for Brain Research, MIT, Cambridge, MA, USA.
Joyce WangThe Picower Institute for Learning and Memory, Department of Brain and Cognitive Sciences, MIT, Cambridge, MA 02139, USA.
Nikita PakThe MIT Media Laboratory, Massachusetts Institute of Technology (MIT), Cambridge, MA, USA; MIT Center for Neurobiological Engineering, MIT, Cambridge, MA, USA; MIT McGovern Institute for Brain Research, MIT, Cambridge, MA, USA; Department of Mechanical Engineering, MIT, Cambridge, MA, USA.
Young-Gyu YoonThe MIT Media Laboratory, Massachusetts Institute of Technology (MIT), Cambridge, MA, USA; MIT Center for Neurobiological Engineering, MIT, Cambridge, MA, USA; MIT McGovern Institute for Brain Research, MIT, Cambridge, MA, USA; Department of Electrical Engineering and Computer Science, MIT, Cambridge, MA, USA; School of Electrical Engineering, KAIST Institute for Health Science and Technology, Daejeon, Republic of Korea.
Jeremy F P UllmannEpilepsy Genetics Program, Department of Neurology, Boston Children's Hospital & Harvard Medical School, Boston, MA, USA.
Burcu Guner-AtamanThe MIT Media Laboratory, Massachusetts Institute of Technology (MIT), Cambridge, MA, USA.
Habiba NoamanyThe Picower Institute for Learning and Memory, Department of Brain and Cognitive Sciences, MIT, Cambridge, MA 02139, USA.
Zoe R SheinkopfThe MIT Media Laboratory, Massachusetts Institute of Technology (MIT), Cambridge, MA, USA; Department of Neurology, Brigham and Women's Hospital, Boston, MA, USA.
Won Min ParkDepartment of Biology, MIT, Cambridge, MA, USA.
Shoh AsanoInternal Medicine Research Unit, Pfizer, Cambridge, MA, USA.
Amy E KeatingDepartment of Biological Engineering, MIT, Cambridge, MA, USA; Department of Biology, MIT, Cambridge, MA, USA; Koch Institute, MIT, Cambridge, MA 02139, USA.
James S TrimmerDepartment of Physiology and Membrane Biology, University of California, Davis School of Medicine, Davis, CA, USA.
Jacob ReimerDepartment of Neuroscience, Baylor College of Medicine, Houston, TX, USA; Center for Neuroscience and AI, Baylor College of Medicine, Houston, TX, USA.
Andreas S ToliasDepartment of Neuroscience, Baylor College of Medicine, Houston, TX, USA; Center for Neuroscience and AI, Baylor College of Medicine, Houston, TX, USA; Department of Electrical and Computer Engineering, Rice University, Houston, TX, USA.
Mark F BearThe Picower Institute for Learning and Memory, Department of Brain and Cognitive Sciences, MIT, Cambridge, MA 02139, USA.
Kay M TyeThe Picower Institute for Learning and Memory, Department of Brain and Cognitive Sciences, MIT, Cambridge, MA 02139, USA; Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
Xue HanBoston University, Department of Biomedical Engineering, Boston, MA 02215, USA.
Misha B AhrensJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
Edward S BoydenThe MIT Media Laboratory, Massachusetts Institute of Technology (MIT), Cambridge, MA, USA; Department of Biological Engineering, MIT, Cambridge, MA, USA; MIT Center for Neurobiological Engineering, MIT, Cambridge, MA, USA; Department of Brain and Cognitive Sciences, MIT, Cambridge, MA, USA; MIT McGovern Institute for Brain Research, MIT, Cambridge, MA, USA; Koch Institute, MIT, Cambridge, MA 02139, USA. Electronic address: edboyden@mit.edu.
IIT@MIT · USMassachusetts Institute of Technology · USBoston University · USMcGovern Institute for Brain Research · USJanelia Research Campus · USBaylor College of Medicine · USBrigham and Women's Hospital · USHarvard University · USHoward Hughes Medical Institute · USPfizer (United States) · USRice University · USSalk Institute for Biological Studies · USUniversity of California, Davis · USVanderbilt University · US
Funding
Recombinant Immunolabels for Nanoprecise Brain Mapping Across ScalesU24NS109113 · NINDS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI KARL Daniel MURRAY · 2018 to 2026
$9.9MNovel Platforms for Systematic Optical Control of Complex Neural Circuits In VivoR01DA029639 · NIDA · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI BOYDEN, EDWARD S. · 2010 to 2023
$6.7MNeural Circuit Mechanisms of Social Homeostasis in Individuals and Supraorganismal Social GroupsDP1AT009925 · NCCIH · SALK INSTITUTE FOR BIOLOGICAL STUDIES · PI TYE, KAY MAXINE · 2017 to 2021
$6.4MRedesigning General Anesthesia (Admin Supp)R01GM104948 · NIGMS · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI BOYDEN, EDWARD S., BROWN, EMERY N · 2012 to 2016
$4.4MMillisecond-Timescale Whole-Brain Neural Activity Mapping in Health and DiseaseDP1NS087724 · NINDS · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI BOYDEN, EDWARD S. · 2013 to 2017
$3.9MHigh-Performance Imaging Through Scattering Living TissueR01DA045549 · NIDA · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI BOYDEN, EDWARD S. · 2017 to 2020
$3.5MGenetically Encoded Localization Modules for Targeting Activity Probes to Specific Subcellular Sites in Brain NeuronsU01NS099714 · NINDS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI TRIMMER, JAMES S · 2016 to 2018
$3.4MRNA Scaffolds for Cell Specific Multiplexed Neural ObservationR01MH114031 · NIMH · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI BOYDEN, EDWARD S. · 2017 to 2020
$2.8MDissecting the Neural Circuits Encoding Positive and Negative ValenceR01MH102441 · NIMH · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI TYE, KAY MAXINE · 2014 to 2018
$2.0MOpto-Crown: Transparent skulls with embedded optics for cortex-wide cellular resolution imaging in freely moving miceRF1NS113287 · NINDS · UNIVERSITY OF MINNESOTA · PI BOYDEN, EDWARD S., KODANDARAMAIAH, SUHASA B · 2019 to 2019
$2.0MVascular Interfaces for Brain Imaging and StimulationR24MH106075 · NIMH · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI DESIMONE, ROBERT · 2014 to 2016
$1.4MA next-generation spatial light modulator for mapping of neural networksR44EB021054 · NIBIB · BOULDER NONLINEAR SYSTEMS, INC. · PI HOY, CHRISTOPHER LUK · 2016 to 2017
$993kHoward Hughes Medical InstituteNCCIH NIH HHS DP1 AT009925NIBIB NIH HHS R44 EB021054NIDA NIH HHS K99 DA045103NIDA NIH HHS R00 DA045103NIDA NIH HHS R01 DA029639NIDA NIH HHS R01 DA045549NIGMS NIH HHS R01 GM104948NIMH NIH HHS F32 MH111216NIMH NIH HHS R01 MH102441NIMH NIH HHS R01 MH114031NIMH NIH HHS R24 MH106075NINDS NIH HHS DP1 NS087724NINDS NIH HHS RF1 NS113287NINDS NIH HHS U01 NS099714NINDS NIH HHS U24 NS109113
Abstract
Methods for one-photon fluorescent imaging of calcium dynamics can capture the activity of hundreds of neurons across large fields of view at a low equipment complexity and cost. In contrast to two-photon methods, however, one-photon methods suffer from higher levels of crosstalk from neuropil, resulting in a decreased signal-to-noise ratio and artifactual correlations of neural activity. We address this problem by engineering cell-body-targeted variants of the fluorescent calcium indicators GCaMP6f and GCaMP7f. We screened fusions of GCaMP to natural, as well as artificial, peptides and identified fusions that localized GCaMP to within 50 μm of the cell body of neurons in mice and larval zebrafish. One-photon imaging of soma-targeted GCaMP in dense neural circuits reported fewer artifactual spikes from neuropil, an increased signal-to-noise ratio, and decreased artifactual correlation across neurons. Thus, soma-targeting of fluorescent calcium indicators facilitates usage of simple, powerful, one-photon methods for imaging neural calcium dynamics.
Indexed as
AnimalsArtifactsBrainCalciumCalcium-Binding ProteinsCell BodyGreen Fluorescent ProteinsMiceNeuronsNeuropilOptical ImagingZebrafishCalciumCalcium-Binding ProteinsGreen Fluorescent Proteinscalcium imagingcorrelationcrosstalkGCaMP6GCaMP7in vivo imagingmicroscopyneuropil contaminationsoma-targetingtwo-photon microscopy
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