ArticleJournal of orthopaedic research : official publication of the Orthopaedic Research Society2021
An ex vivo human osteochondral culture model.
Article in Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 23 citations in OpenAlex.
- A Pre-Clinical Model of Synovitis Using Ex vivo Human Synovial Tissue with Preserved Function and Architecture.Journal of visualized experiments : JoVE · 2026Article
- A disease-severity-responsive nanoparticle enables potent ghrelin messenger RNA therapy in osteoarthritis.Nature nanotechnology · 2026Article
- Review
- Evaluation of MSC-Secretome Effects in an Ex Vivo Compartmentalized Osteochondral Interface Model.Stem cells international · 2026Article
- Development of an Ex Vivo Osteochondral Biomimetic Platform for Mechanistic Investigation of Cartilage Regeneration.International journal of molecular sciences · 2025Article
- Modern Approach to Testing the Biocompatibility of Osteochondral Scaffolds in Accordance with the 3Rs Principle─PreclinicalACS biomaterials science & engineering · 2025Article
- Culture system for longitudinal monitoring of bone dynamics ex vivo.Biotechnology and bioengineering · 2025Article
- Magnetically Actuated Soft Electrodes for Multisite Bioelectrical Monitoring of Ex Vivo Tissues.Cyborg and bionic systems (Washington, D.C.) · 2025Article
- Animal Models of Osteoarthritis: Updated Models and Outcome Measures 2016-2023.Regenerative engineering and translational medicine · 2024Article
- The effect of multi-material architecture on the ex vivo osteochondral integration of bioprinted constructs.Acta biomaterialia · 2023Article
- Human Osteochondral Explants as an Ex Vivo Model of Osteoarthritis for the Assessment of a Novel Class of Orthobiologics.Pharmaceutics · 2022Article
- Joint-on-chip platforms: entering a new era of in vitro models for arthritis.Nature reviews. Rheumatology · 2022Review
- An ex vivo human osteochondral culture model.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2021Article
- Ex Vivo Systems to Study Chondrogenic Differentiation and Cartilage Integration.Journal of functional morphology and kinesiology · 2021Review
- Establishment of anFrontiers in bioengineering and biotechnology · 2021Article
- Biofabrication of the osteochondral unit and its applications: Current and future directions for 3D bioprinting.Journal of tissue engineeringReview
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To reduce animal experimentation and to overcome translational issues in cartilage tissue engineering, there is a need to develop an ex vivo human tissue-based approach. This study aims to demonstrate that a human osteochondral explant at different stages of osteoarthritis (OA) can be kept in long-term culture while preserving its viability and composition. Osteochondral explants with either a smooth or fibrillated cartilage surface, representing different OA stages, were harvested from fresh human tibial plateaus. Explants were cultured for 2 or 4 weeks in a double-chamber culture platform. The biochemical content of the cartilage of cultured explants did not significantly change over a period of 4 weeks and these findings were supported by histology. Chondrocytes mostly preserved their metabolic activity during culture and active bone and marrow were found in the periphery of the explants, while metabolic activity was decreased in the bone core in cultured explants compared to fresh explants. In fibrillated explants, chondrocyte viability decreased in the periphery of the sample in cultured groups compared to fresh explants (fresh, 94 ± 6%; cultured, 64% ± 17%, 2 weeks, and 69% ± 17%, 4 weeks; P < .05). Although biochemical and histological results did not show changes within the cartilage tissue, the viability of the explants should be carefully controlled for each specific use. This system provides an alternative to explore drug treatment and implant performance under more controlled experimental conditions than possible in vivo, in combination with clinically relevant human osteochondral tissue.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.