ArticleScientific reports2020
LoTToR: An Algorithm for Missing-Wedge Correction of the Low-Tilt Tomographic 3D Reconstruction of a Single-Molecule Structure.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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24 citing papers in PubMed, 43 citations in OpenAlex.
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- ChromEMT: visualizing and reconstructing chromatin ultrastructure and 3D organization in situ.Nature protocols · 2025Review
- Non-averaged single-molecule tertiary structures reveal RNA self-folding through individual-particle cryo-electron tomography.Nature communications · 2024Article
- Correction of preferred orientation-induced distortion in cryo-electron microscopy maps.Science advances · 2024Article
- Angle between DNA linker and nucleosome core particle regulates array compaction revealed by individual-particle cryo-electron tomography.Nature communications · 2024Article
- The Essence of Lipoproteins in Cardiovascular Health and Diseases Treated by Photodynamic Therapy.Biomedicines · 2024Review
- DUAL: deep unsupervised simultaneous simulation and denoising for cryo-electron tomography.bioRxiv : the preprint server for biology · 2024Article
- SINGLE MOLECULE 3D STRUCTURES DETERMINED BY INDIVIDUAL-PARTICLE ELECTRON TOMOGRAPHY.iScience notes · 2024Article
- Direct in situ measurements of electrical properties of solid-electrolyte interphase on lithium metal anodes.Nature energy · 2023Article
- Genetically encoded barcodes for correlative volume electron microscopy.Nature biotechnology · 2023Article
- Insights into the 3D permeable pore structure within novel monodisperse mesoporous silica nanoparticles by cryogenic electron tomography.Nanoscale advances · 2023Article
- Designer molecules of the synaptic organizer MDGA1 reveal 3D conformational control of biological function.The Journal of biological chemistry · 2023Article
- Structure-based mechanism and inhibition of cholesteryl ester transfer protein.Current atherosclerosis reports · 2023Review
- Computational methods forFrontiers in cellular and infection microbiology · 2023Review
- Isotropic reconstruction for electron tomography with deep learning.Nature communications · 2022Article
- Molecular organization of the early stages of nucleosome phase separation visualized by cryo-electron tomography.Molecular cell · 2022Article
- Structure Detection in Three-Dimensional Cellular Cryoelectron Tomograms by Reconstructing Two-Dimensional Annotated Tilt Series.Journal of computational biology : a journal of computational molecular cell biology · 2022Article
- Cryo-electron tomography related radiation-damage parameters for individual-molecule 3D structure determination.Frontiers in chemistry · 2022Review
- Deep Learning-Based Image Reconstruction for Different Medical Imaging Modalities.Computational and mathematical methods in medicine · 2022Review
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
A single-molecule three-dimensional (3D) structure is essential for understanding the thermal vibrations and dynamics as well as the conformational changes during the chemical reaction of macromolecules. Individual-particle electron tomography (IPET) is an approach for obtaining a snap-shot 3D structure of an individual macromolecule particle by aligning the tilt series of electron tomographic (ET) images of a targeted particle through a focused iterative 3D reconstruction method. The method can reduce the influence on the 3D reconstruction from large-scale image distortion and deformation. Due to the mechanical tilt limitation, 3D reconstruction often contains missing-wedge artifacts, presented as elongation and an anisotropic resolution. Here, we report a post-processing method to correct the missing-wedge artifact. This low-tilt tomographic reconstruction (LoTToR) method contains a model-free iteration process under a set of constraints in real and reciprocal spaces. A proof of concept is conducted by using the LoTToR on a phantom, i.e., a simulated 3D reconstruction from a low-tilt series of images, including that within a tilt range of ±15°. The method is validated by using both negative-staining (NS) and cryo-electron tomography (cryo-ET) experimental data. A significantly reduced missing-wedge artifact verifies the capability of LoTToR, suggesting a new tool to support the future study of macromolecular dynamics, fluctuation and chemical activity from the viewpoint of single-molecule 3D structure determination.
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