ArticleNature communications2020
Tracking down the molecular architecture of the synaptonemal complex by expansion microscopy.
Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT07639359 (Ketamine In Depression - Intensive Care Unit Trial), which is not on this map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Ketamine In Depression - Intensive Care Unit Trial (KID-ICU): A Phase II Randomized, Double-Blind, Placebo-Controlled Multicenter Study of Ketamine Infusion for Depressive Symptoms in Intensive Care Unit Patients
Who cites it
33 citing papers in PubMed, 52 citations in OpenAlex.
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- Structure and function of the synaptonemal complex.The Journal of cell biology · 2026Review
- Review of expansion microscopy combined with advanced imaging modalities.Journal of microscopy · 2026Review
- Minor spliceosome inhibition via ablation of its U11 snRNA gene results in multiple defects in murine spermatogenesis.Nucleic acids research · 2026Article
- Dual-Color Expansion Microscopy of Membrane Proteins Using Bioorthogonal Labeling.Nano letters · 2026Article
- Spatial mapping of DNA synthesis reveals dynamics and geometry of human replication nanostructures.The EMBO journal · 2025Article
- Multi-step implementation of meiotic crossover patterning.bioRxiv : the preprint server for biology · 2025Article
- Chromosomal Roadblocks in Male Fertility: Mechanisms, Risk Factors and Syndromes.Medicina (Kaunas, Lithuania) · 2025Review
- Deciphering meiotic chromatin organization by SYCP3.Nucleic acids research · 2025Article
- Crossover recombination between homologous chromosomes in meiosis: recent progress and remaining mysteries.Trends in genetics : TIG · 2025Review
- GPR180 is a new member of the Golgi-dynamics domain seven-transmembrane helix protein family.Communications biology · 2024Article
- Expanding Insights: Harnessing Expansion Microscopy for Super-Resolution Analysis of HIV-1-Cell Interactions.Viruses · 2024Article
- LGI1 Autoantibodies Enhance Synaptic Transmission by Presynaptic KNeurology(R) neuroimmunology & neuroinflammation · 2024Article
- Formation and resolution of meiotic chromosome entanglements and interlocks.Journal of cell science · 2024Review
- Meiosis through three centuries.Chromosoma · 2024Review
- Enhanced synaptic protein visualization by multicolor super-resolution expansion microscopy.Neurophotonics · 2023Article
- Cohesin-mediated DNA loop extrusion resolves sister chromatids in G2 phase.The EMBO journal · 2023Article
- Micro Germline-Restricted Chromosome in Blue Tits: Evidence for Meiotic Functions.Molecular biology and evolution · 2023Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
The synaptonemal complex (SC) is a meiosis-specific nuclear multiprotein complex that is essential for proper synapsis, recombination and segregation of homologous chromosomes. We combined structured illumination microscopy (SIM) with different expansion microscopy (ExM) protocols including U-ExM, proExM, and magnified analysis of the proteome (MAP) to investigate the molecular organization of the SC. Comparison with structural data obtained by single-molecule localization microscopy of unexpanded SCs allowed us to investigate ultrastructure preservation of expanded SCs. For image analysis, we developed an automatic image processing software that enabled unbiased comparison of structural properties pre- and post-expansion. Here, MAP-SIM provided the best results and enabled reliable three-color super-resolution microscopy of the SCs of a whole set of chromosomes in a spermatocyte with 20-30 nm spatial resolution. Our data demonstrate that post-expansion labeling by MAP-SIM improves immunolabeling efficiency and allowed us thus to unravel previously hidden details of the molecular organization of SCs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.