Evidence map›Paper›PMID 32590007›Full record

ArticleThe Journal of infection2020

Molecular identification of Cryptococcus gattii from cerebrospinal fluid using single-cell sequencing: A case study.

Min Chen, Nan Hong, Shan Hu, Peng Wang, HongZhi Guan, Meng Xiao, Xinlin Zhu, Abdullah M S Al-Hatmi, Zhe Zhou, Lei Gao and 5 more

Open access · bronzeAbstract readCase Reports
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of infection, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
1.3field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 12 citations in OpenAlex.

  1. Molecular Diagnostics ofDiseases (Basel, Switzerland) · 2024
    Review
  2. Review
  3. Article
  4. Review
  5. A SERS Platform for Rapid Detection of Drug Resistance of Non-International journal of nanomedicine · 2022
    Article
  6. Article
  7. Review
  8. Review
  9. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 8 institutions in 4 countries.

Min ChenBeijing Key Laboratory of New Molecular Diagnosis Technologies for Infectious Disease, Department of Biotechnology, Beijing Institute of Radiation Medicine, Beijing, China; Shanghai Key Laboratory of Molecular Medical Mycology, Department of Dermatology, Changzheng Hospital, Second Military Medical University, Shanghai, China.
Nan HongShanghai Key Laboratory of Molecular Medical Mycology, Department of Dermatology, Changzheng Hospital, Second Military Medical University, Shanghai, China.
Shan HuBeijing Key Laboratory of New Molecular Diagnosis Technologies for Infectious Disease, Department of Biotechnology, Beijing Institute of Radiation Medicine, Beijing, China; Department of Laboratory Medicine, Xuzhou Tumor Hospital, Xuzhou, China.
Peng WangDepartment of Clinical Laboratory, and Beijing Key Laboratory for Mechanisms Research and Precision Diagnosis of Invasive Fungal Diseases, Peking Union Medical College Hospital, Beijing, China.
HongZhi GuanDepartment of Neurology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing, China.
Meng XiaoDepartment of Clinical Laboratory, and Beijing Key Laboratory for Mechanisms Research and Precision Diagnosis of Invasive Fungal Diseases, Peking Union Medical College Hospital, Beijing, China.
Xinlin ZhuShanghai Key Laboratory of Molecular Medical Mycology, Department of Dermatology, Changzheng Hospital, Second Military Medical University, Shanghai, China.
Abdullah M S Al-HatmiMinistry of Health, Directorate General of Health Services, Ibri, Oman.
Zhe ZhouBeijing Key Laboratory of New Molecular Diagnosis Technologies for Infectious Disease, Department of Biotechnology, Beijing Institute of Radiation Medicine, Beijing, China.
Lei GaoMicroscopy Core Facility, Biomedical Research Core Facilities, Westlake University, Hangzhou, China.
Teun BoekhoutWesterdijk Fungal Biodiversity Institute, Utrecht, the Netherlands; Institute of Biodiversity and Ecosystem Dynamics, University of Amsterdam, Amsterdam, the Netherlands.
Jianping XuDepartment of Biology, McMaster University, Hamilton, Canada.
Yingchun XuDepartment of Clinical Laboratory, and Beijing Key Laboratory for Mechanisms Research and Precision Diagnosis of Invasive Fungal Diseases, Peking Union Medical College Hospital, Beijing, China. Electronic address: xycpumch@139.com.
Wanqing LiaoShanghai Key Laboratory of Molecular Medical Mycology, Department of Dermatology, Changzheng Hospital, Second Military Medical University, Shanghai, China. Electronic address: liaowanqing@smmu.edu.cn.
Ying YangBeijing Key Laboratory of New Molecular Diagnosis Technologies for Infectious Disease, Department of Biotechnology, Beijing Institute of Radiation Medicine, Beijing, China. Electronic address: y_ying_77@163.com.
Second Military Medical University · CNBeijing Radiation Center · CNPeking Union Medical College Hospital · CNChinese Academy of Medical Sciences & Peking Union Medical College · CNMcMaster University · CAMinistry of Health · OMWesterdijk Fungal Biodiversity Institute · NLWestlake University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A 31-year-old man presented with cryptococcal meningitis (CM) without typical clinical characteristics, but with abnormal walking, difficult leg lifting and frequent falling. He was admitted to Peking Union Medical College Hospital. After multiple tests failed to identify the pathogen, single-cell sequencing (scS) was used to test the cerebrospinal fluid (CSF). Comparing the sequence obtained from single-cell sequencing with the reference database, it was found that the infection was caused by Cryptococcus gattii sensu stricto (AFLP4/VGI genotype). Cryptococcus is difficult to cultivate from complex body fluids. The etiological agent of this patient was identified and the patient was treated. This is the first case in which scS was used to detect and identify fungal pathogen after conventional testing failed to identify the cause of the disease. This report demonstrates that the scS approach can be used to generate fungal genome sequences directly from the CSF of a CM patient. The scS technology could become a powerful tool to precise detect microscopically visible but uncultured pathogens in clinical samples.

Indexed as

CryptococcosisCryptococcus gattiiCryptococcus neoformansMeningitis, CryptococcalAdultGenotypeHumansMaleCryptococcal meningitisCryptococcus gattiiCulture-independent molecular diagnosisSingle-cell sequencing

Identifiers

PMID32590007
PMCPMC7309800
OpenAlexW3037046972

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.