ArticleAngewandte Chemie (International ed. in English)2020
Insect-Associated Bacteria Assemble the Antifungal Butenolide Gladiofungin by Non-Canonical Polyketide Chain Termination.
Article in Angewandte Chemie (International ed. in English), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 48 citations in OpenAlex.
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- Genome minimization of a Burkholderia bacterial host.Metabolic engineering · 2026Article
- Identifying phenotypic and genetic traits for assessing pathogenic potential and biocontrol capacity in Burkholderia sensu lato strains.The ISME journal · 2026Article
- Ecological Diversity, Metabolic Versatility, and Biotechnological Applications ofAntibiotics (Basel, Switzerland) · 2025Review
- Research progress of glutarimide-containing polyketides: Structures, bioactivities and their biosynthesis.Pharmaceutical science advances · 2025Review
- Thiotemplated Polyketide Chain Fusion and Reductive Cyclization Build the Reactive Butenolide Core of Malleicyprol.Angewandte Chemie (International ed. in English) · 2025Article
- Microbial associates of the elm leaf beetle: uncovering the absence of resident bacteria and the influence of fungi on insect performance.Applied and environmental microbiology · 2024Article
- Deazaflavin metabolite produced by endosymbiotic bacteria controls fungal host reproduction.The ISME journal · 2024Article
- The Genomic-Driven Discovery of Glutarimide-Containing Derivatives fromMolecules (Basel, Switzerland) · 2023Article
- Compilation of the Antimicrobial Compounds Produced byMolecules (Basel, Switzerland) · 2023Review
- Genomic and phenotypic characterization of a red-pigmented strain ofFrontiers in microbiology · 2023Article
- Insights into group-specific pattern of secondary metabolite gene cluster inFrontiers in microbiology · 2023Review
- Synthesis and Antifungal Activity of New butenolide Containing Methoxyacrylate Scaffold.Molecules (Basel, Switzerland) · 2022Article
- Biotechnology approaches for natural product discovery, engineering, and production based on Burkholderia bacteria.Current opinion in biotechnology · 2022Review
- Deciphering Chemical Mediators Regulating Specialized Metabolism in a Symbiotic Cyanobacterium.Angewandte Chemie (International ed. in English) · 2022Article
- Article
- Biosynthesis of Sinapigladioside, an Antifungal Isothiocyanate from Burkholderia Symbionts.Chembiochem : a European journal of chemical biology · 2021Article
- Article
- Phylogenetic analysis of the salinipostin γ-butyrolactone gene cluster uncovers new potential for bacterial signalling-molecule diversity.Microbial genomics · 2021Article
- Genomics-Driven Activation of Silent Biosynthetic Gene Clusters inMolecules (Basel, Switzerland) · 2021Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genome mining of one of the protective symbionts (Burkholderia gladioli) of the invasive beetle Lagria villosa revealed a cryptic gene cluster that codes for the biosynthesis of a novel antifungal polyketide with a glutarimide pharmacophore. Targeted gene inactivation, metabolic profiling, and bioassays led to the discovery of the gladiofungins as previously-overlooked components of the antimicrobial armory of the beetle symbiont, which are highly active against the entomopathogenic fungus Purpureocillium lilacinum. By mutational analyses, isotope labeling, and computational analyses of the modular polyketide synthase, we found that the rare butenolide moiety of gladiofungins derives from an unprecedented polyketide chain termination reaction involving a glycerol-derived C3 building block. The key role of an A-factor synthase (AfsA)-like offloading domain was corroborated by CRISPR-Cas-mediated gene editing, which facilitated precise excision within a PKS domain.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.