Evidence map›Paper›PMID 32588946›Full record

ArticleCell proliferation2020

Bone mesenchymal stem cells are recruited via CXCL8-CXCR2 and promote EMT through TGF-β signal pathways in oral squamous carcinoma.

Lin Meng, Yueqi Zhao, Wenhuan Bu, Xing Li, Xinchen Liu, Dabo Zhou, Yumeng Chen, Shize Zheng, Quan Lin, Qilin Liu and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell proliferation, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed, 1 pooled it
1.8field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed, 1 synthesis or guideline pooled it, 38 citations in OpenAlex.

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  8. [Research progress on bone repair biomaterials with the function of recruiting endogenous mesenchymal stem cells].Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

Lin MengDepartment of Oral Pathology, Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, School and Hospital of Stomatology, Jilin University, Changchun, China.ORCID https://orcid.org/0000-0002-7969-9488
Yueqi ZhaoState Key Laboratory of Supramolecular Structure and Material, College of Chemistry, Jilin University, Changchun, China.
Wenhuan BuSchool and Hospital of Stomatology, China Medical University, Shenyang, China.
Xing LiSchool and Hospital of Stomatology, China Medical University, Shenyang, China.
Xinchen LiuDepartment of Oral Pathology, Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, School and Hospital of Stomatology, Jilin University, Changchun, China.
Dabo ZhouSchool and Hospital of Stomatology, China Medical University, Shenyang, China.
Yumeng ChenDepartment of Oral Pathology, Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, School and Hospital of Stomatology, Jilin University, Changchun, China.
Shize ZhengDepartment of Oral Pathology, Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, School and Hospital of Stomatology, Jilin University, Changchun, China.
Quan LinState Key Laboratory of Supramolecular Structure and Material, College of Chemistry, Jilin University, Changchun, China.
Qilin LiuDepartment of Oral Pathology, Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, School and Hospital of Stomatology, Jilin University, Changchun, China.
Hongchen SunDepartment of Oral Pathology, Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, School and Hospital of Stomatology, Jilin University, Changchun, China.ORCID https://orcid.org/0000-0002-5572-508X
Jilin University · CNChina Medical University · CN

Funding

China Postdoctoral Science Foundation 2019M661177Jilin Scientific and Technological Development Program 20170101093JCJilin University 2015340National Key R&D Program of China 2016YFC1102800National Natural Science Foundation of China 81320108011National Natural Science Foundation of China 81870741National Natural Science Foundation of China 81920108012Provincial Industrial Innovation Project of Jilin Province 2016C044-3State Key Laboratory of Luminescence and Applications SKLA-2020-07
6 · The paper itself

Abstract

objectivesBone mesenchymal stem cells (BMSCs) play critical roles in tumour microenvironment. However, molecular mechanisms of how BMSCs to be recruited and effect subsequent tumour progression are poorly understood in oral squamous cell carcinoma (OSCC). MATERIALS AND

methodsThe distribution of CXCL8 was detected by immunohistochemical staining in OSCC tissues. The chemotaxis of conditioned media from different epithelial cells to BMSCs was examined by trans-well assay. Real-time quantitative PCR (qPCR) and ELISA were used to detect the expression of related cytokines and chemokine receptors. The migration of BMSCs was observed in BALB/c nude mice. The roles of BMSCs in proliferation, migration and invasion of OSCC were detected by CCK-8, flow cytometry and trans-well assay. Epithelial-mesenchymal transition (EMT)-related markers were analysed by qPCR and Western blot in vitro, and growth was evaluated in BALB/c nude mice using subcutaneously implanted OSCC in nude mouse model in vivo.

resultsUsing OSCC, we show CXCL8, secreted by OSCC, binds to exclusively CXCR2 in BMSCs to facilitate migration of BMSCs to OSCC. TGF-β secreted by BMSCs subsequently induces EMT of OSCC to promote their proliferation, migration and infiltration. We also showed that the Ras/Raf/Erk axis plays a critical role in tumour progression.

conclusionsOur results provide the molecular basis for BMSC recruitment into tumours, and how this process leads to tumour progression and leads us to develop a novel OSCC treatment target.

Indexed as

Epithelial-Mesenchymal TransitionSignal TransductionAnimalsCadherinsCarcinoma, Squamous CellCell MovementCell ProliferationGene Expression Regulation, NeoplasticInterleukin-8MaleMesenchymal Stem CellsMice, NudeMouth NeoplasmsReceptors, Interleukin-8BTransforming Growth Factor betaTumor MicroenvironmentCadherinsCxcr2 protein, mouseInterleukin-8Receptors, Interleukin-8BTransforming Growth Factor betabone mesenchymal stem cellschemotaxisCXCL8epithelial-mesenchymal transitionoral squamous cell carcinomaTGF-β/Ras/Raf/Erk

Identifiers

PMID32588946
PMCPMC7445409
OpenAlexW3037226990

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.