Evidence map›Paper›PMID 32588241›Full record

ArticleArchives of virology2020

Identification of proteins associated with two diverse Caulobacter phicbkvirus particles.

Kiesha Wilson, Fanchao Zhu, Ran Zheng, Sixue Chen, Bert Ely

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Archives of virology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 95% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Kiesha WilsonDepartment of Biological Sciences, University of South Carolina, Columbia, SC, 29208, USA.
Fanchao ZhuInterdisciplinary Center for Biotechnology Research, University of Florida, Gainesville, FL, 32610, USA.
Ran ZhengInterdisciplinary Center for Biotechnology Research, University of Florida, Gainesville, FL, 32610, USA.
Sixue ChenInterdisciplinary Center for Biotechnology Research, University of Florida, Gainesville, FL, 32610, USA.
Bert ElyDepartment of Biological Sciences, University of South Carolina, Columbia, SC, 29208, USA. ely@sc.edu.ORCID http://orcid.org/0000-0002-2768-2940
University of Florida · USUniversity of South Carolina · US

Funding

UofSC Postbaccalaureate Research Education ProgramR25GM066526 · NIGMS · UNIVERSITY OF SOUTH CAROLINA AT COLUMBIA · PI ARMSTRONG, ALISSA RICHMOND, ELY, BERT · 2004 to 2024
$5.4M
University of South Carolina Initiative for Maximizing Student DevelopmentR25GM076277 · NIGMS · UNIVERSITY OF SOUTH CAROLINA AT COLUMBIA · PI ELY, BERT · 2006 to 2015
$2.6M
NIGMS NIH HHS GM076277NIGMS NIH HHS R25 GM066526NIGMS NIH HHS R25 GM076277
6 · The paper itself

Abstract

Genomic evolution among bacteriophages infecting Caulobacter crescentus is inevitable. However, the conservation of the proteins associated with intact phage particles has not been investigated. In this study, we compared the structural proteins associated with two genomically diverse but morphologically similar C. crescentus-infecting bacteriophages, phiCbK and CcrSC. We were able to detect more than 20 proteins that are part of the bacteriophage particle in both phages, and we were able to identify a small number of proteins that were found in only one of the two phage particles. All but one of the genes coding for these structural proteins were located in a region of the genome that had been designated a structural region, confirming the idea that the genes in these phage genomes are clustered according to their function. During the purification process, we also discovered that phiCbk has a replication complex that can be recovered from the cell lysate, and this complex allowed us to identify many of the phage proteins involved in phage genome replication.

Indexed as

BacteriophagesCaulobacter crescentusGenome, ViralGenomicsSiphoviridaeViral ProteinsViral Proteins

Identifiers

PMID32588241
PMCPMC7415651
OpenAlexW3037099303

What OpenQuestion holds

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LicenceTDM
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.