Evidence map›Paper›PMID 32587965›Full record

ArticlebioRxiv : the preprint server for biology2020

Specific viral RNA drives the SARS CoV-2 nucleocapsid to phase separate.

Christiane Iserman, Christine Roden, Mark Boerneke, Rachel Sealfon, Grace McLaughlin, Irwin Jungreis, Chris Park, Avinash Boppana, Ethan Fritch, Yixuan J Hou and 6 more

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 57 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 4 institutions in 1 country.

Christiane IsermanDepartment of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Christine RodenDepartment of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Mark BoernekeDepartment of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Rachel SealfonFlatiron Institute, Simons Foundation, New York, NY, USA.
Grace McLaughlinDepartment of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Irwin JungreisBroad Institute, MIT, Cambridge, MA, USA.
Chris ParkFlatiron Institute, Simons Foundation, New York, NY, USA.
Avinash BoppanaDepartment of Computer Science, Princeton University, Princeton, NJ USA.
Ethan FritchDepartment of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Yixuan J HouDepartment of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Chandra TheesfeldLewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ USA.
Olga G TroyanskayaFlatiron Institute, Simons Foundation, New York, NY, USA.
Ralph S BaricDepartment of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Timothy P SheahanDepartment of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Kevin WeeksDepartment of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Amy S GladfelterDepartment of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
University of North Carolina at Chapel Hill · USPrinceton University · USSimons Foundation · USBroad Institute · US

Funding

Integrated Training in Cancer Model SystemsT32CA009156 · NCI · UNIV OF NORTH CAROLINA CHAPEL HILL · PI DER, CHANNING J. · 1985 to 2020
$19.7M
Pilot Study: POPULATION PHARMACOLOGYU54HL117798 · NHLBI · UNIVERSITY OF PENNSYLVANIA · PI FITZGERALD, GARRET A · 2012 to 2016
$18.5M
lntegration and Visualization of Diverse Biological DataR01GM071966 · NIGMS · PRINCETON UNIVERSITY · PI TROYANSKAYA, OLGA G · 2005 to 2022
$6.4M
Discovery and Function of Higher-Order RNA StructureR35GM122532 · NIGMS · UNIV OF NORTH CAROLINA CHAPEL HILL · PI Kevin M Weeks · 2017 to 2026
$5.7M
Cytoplasmic Organization by phase transitionsR01GM081506 · NIGMS · UNIV OF NORTH CAROLINA CHAPEL HILL · PI GLADFELTER, AMY S · 2010 to 2023
$4.0M
Context-Sensitive Search of Human Expression CompendiaR01HG005998 · NHGRI · PRINCETON UNIVERSITY · PI TROYANSKAYA, OLGA G · 2011 to 2013
$1.1M
RNA structures critical for dictating the contents, behavior, and function of droplets formed from liquid-liquid phase separationF32GM136164 · NIGMS · UNIV OF NORTH CAROLINA CHAPEL HILL · PI RODEN, CHRISTINE ANNE · 2020 to 2022
$157k
The role of higher order RNA structure in RNA virus replication and infectionF32GM128330 · NIGMS · UNIV OF NORTH CAROLINA CHAPEL HILL · PI BOERNEKE, MARK A · 2018 to 2019
$120k
NCI NIH HHS T32 CA009156NHGRI NIH HHS R01 HG005998NHLBI NIH HHS U54 HL117798NIAID NIH HHS HHSN272201000054CNIGMS NIH HHS F32 GM128330NIGMS NIH HHS F32 GM136164NIGMS NIH HHS R01 GM071966NIGMS NIH HHS R01 GM081506NIGMS NIH HHS R35 GM122532
6 · The paper itself

Abstract

A mechanistic understanding of the SARS-CoV-2 viral replication cycle is essential to develop new therapies for the COVID-19 global health crisis. In this study, we show that the SARS-CoV-2 nucleocapsid protein (N-protein) undergoes liquid-liquid phase separation (LLPS) with the viral genome, and propose a model of viral packaging through LLPS. N-protein condenses with specific RNA sequences in the first 1000 nts (5'-End) under physiological conditions and is enhanced at human upper airway temperatures. N-protein condensates exclude non-packaged RNA sequences. We comprehensively map sites bound by N-protein in the 5'-End and find preferences for single-stranded RNA flanked by stable structured elements. Liquid-like N-protein condensates form in mammalian cells in a concentration-dependent manner and can be altered by small molecules. Condensation of N-protein is sequence and structure specific, sensitive to human body temperature, and manipulatable with small molecules thus presenting screenable processes for identifying antiviral compounds effective against SARS-CoV-2.

Indexed as

gRNA packagingnucleocapsidphase separationSARS CoV-2

Identifiers

PMID32587965
PMCPMC7310621
OpenAlexW3035338003

What OpenQuestion holds

Textmetadata
LicenceCC BY-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.