ArticleJournal of veterinary research2020
A Novel, Rapid, and Simple PMA-qPCR Method for Detection and Counting of Viable
Article in Journal of veterinary research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
11 citing papers in PubMed, 17 citations in OpenAlex.
- Application-focused validation of a viability-selective PMA-qPCR assay for selected skin-associated and cosmetic-relevant bacteria.Frontiers in microbiology · 2026Article
- Optimization ofJournal of environmental engineering (New York, N.Y.) · 2025Article
- Specific detection of Brucella spp. from slaughter-aged livestock using a dual priming oligonucleotide system.BMC microbiology · 2025Article
- A recombinase polymerase amplification-SYBR Green I assay for the rapid and visual detection of Brucella.Folia microbiologica · 2024Article
- Development and validation of a PMA-qPCR method for accurate quantification of viableFrontiers in microbiology · 2024Article
- Topical Administration ofMicroorganisms · 2023Article
- A rapid and reliable method for the determination ofFrontiers in microbiology · 2023Article
- Article
- A Propidium Monoazide (PMAxx)-Droplet Digital PCR (ddPCR) for the Detection of ViableMicroorganisms · 2022Article
- An Assay Combining Droplet Digital PCR With Propidium Monoazide Treatment for the Accurate Detection of Live Cells ofFrontiers in microbiology · 2022Article
- How to Evaluate Non-Growing Cells-Current Strategies for Determining Antimicrobial Resistance of VBNC Bacteria.Antibiotics (Basel, Switzerland) · 2021Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionThe plate counting method widely used at present to discern viable from non-viable MATERIAL AND
methodsUsing propidium monoazide (PMA) to inhibit amplification of DNA from dead
resultsThe optimal exposure time and working concentration of PMA were 10 min and 15 μg/mL, respectively. The correlation coefficient (R
conclusionIn this study, a rapid and simple PMA-qPCR counting method for viable
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.