Evidence map›Paper›PMID 32587369›Full record

ArticleScientific reports2020

TET1 Deficiency Impairs Morphogen-free Differentiation of Human Embryonic Stem Cells to Neuroectoderm.

Hanqin Li, Zhixing Hu, Houbo Jiang, Jiali Pu, Ilana Selli, Jingxin Qiu, Baorong Zhang, Jian Feng

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.8field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. New Insights into TETs in Psychiatric Disorders.International journal of molecular sciences · 2022
    Review
  5. Article
  6. Structure and Function of TET Enzymes.Advances in experimental medicine and biology · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Hanqin LiDepartment of Physiology and Biophysics, State University of New York at Buffalo, Buffalo, NY, 14203, USA.
Zhixing HuDepartment of Physiology and Biophysics, State University of New York at Buffalo, Buffalo, NY, 14203, USA.
Houbo JiangDepartment of Physiology and Biophysics, State University of New York at Buffalo, Buffalo, NY, 14203, USA.
Jiali PuDepartment of Neurology, Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China.
Ilana SelliDepartment of Physiology and Biophysics, State University of New York at Buffalo, Buffalo, NY, 14203, USA.
Jingxin QiuDepartment of Pathology and Laboratory Medicine, Roswell Park Comprehensive Cancer Center, Buffalo, NY, 14263, USA.
Baorong ZhangDepartment of Neurology, Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China.
Jian FengDepartment of Physiology and Biophysics, State University of New York at Buffalo, Buffalo, NY, 14203, USA. jianfeng@buffalo.edu.
University at Buffalo, State University of New York · USSecond Affiliated Hospital of Zhejiang University · CNRoswell Park Comprehensive Cancer Center · US

Funding

A Novel Epigenetic Mechanism for Alzheimer's DiseaseR01AG056060 · NIA · STATE UNIVERSITY OF NEW YORK AT BUFFALO · PI YAN, ZHEN · 2017 to 2021
$2.2M
Functions of parkin in Parkinson’s diseaseR01NS102148 · NINDS · STATE UNIVERSITY OF NEW YORK AT BUFFALO · PI FENG, JIAN · 2018 to 2021
$1.8M
Functions of parkin in Parkinson’s diseaseR56NS102148 · NINDS · STATE UNIVERSITY OF NEW YORK AT BUFFALO · PI FENG, JIAN · 2017 to 2017
$399k
BLRD VA I01 BX003831NIA NIH HHS R01 AG056060NINDS NIH HHS R01 NS102148NINDS NIH HHS R56 NS102148
6 · The paper itself

Abstract

The TET family of 5-methylcytosine (5mC) dioxygenases plays critical roles in development by modifying DNA methylation. Using CRISPR, we inactivated the TET1 gene in H9 human embryonic stem cells (hESCs). Mutant H9 hESCs remained pluripotent, even though the level of hydroxymethylcytosine (5hmC) decreased to 30% of that in wild-type cells. Neural differentiation induced by dual SMAD inhibitors was not significantly affected by loss of TET1 activity. However, in a morphogen-free condition, TET1 deficiency significantly reduced the generation of NESTIN

Indexed as

5-MethylcytosineCell DifferentiationCell LineCRISPR-Cas SystemsDNA MethylationEpigenesis, GeneticFrameshift MutationGene Expression Regulation, DevelopmentalHuman Embryonic Stem CellsHumansMixed Function OxygenasesNeural PlateNeurogenesisNeuronsPAX6 Transcription FactorPromoter Regions, Genetic5-MethylcytosineMixed Function OxygenasesPAX6 protein, humanPAX6 Transcription FactorProto-Oncogene ProteinsSOX1 protein, humanSOXB1 Transcription FactorsTET1 protein, human

Identifiers

PMID32587369
PMCPMC7316867
OpenAlexW3038056967

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.