ArticleMethods in cell biology2020
Generation of esophageal organoids and organotypic raft cultures from human pluripotent stem cells.
Article in Methods in cell biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 16 citations in OpenAlex.
- An organoid-guided roadmap for precision delivery of epigallocatechin gallate in oral submucous fibrosis.Frontiers in bioengineering and biotechnology · 2026Review
- Human pluripotent stem cell-derived organoids repair damaged bowel in vivo.Cell stem cell · 2024Article
- Directed differentiation of human embryonic stem cells into parathyroid cells and establishment of parathyroid organoids.Cell proliferation · 2024Article
- Mammalian esophageal stratified tissue homeostasis is maintained distinctively by the epithelial pluripotent p63Cellular and molecular life sciences : CMLS · 2023Article
- Organotypic 3D Co-Culture of Human Pleura as a Novel In Vitro Model ofBioengineering (Basel, Switzerland) · 2023Article
- Novel 3D organotypic co-culture model of pleura.PloS one · 2022Article
- Developmental basis of trachea-esophageal birth defects.Developmental biology · 2021Review
- Article
- Advances in esophageal organoids: from construction to applications.Journal of tissue engineeringReview
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Authors and funding
2 authors at 1 institution in 1 country.
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Abstract
The human and murine esophagus have some substantial differences that limit the utility of mouse as a model to study human esophagus development and disease. Due to these limitations several recent reports describe the development of methods to generate human esophageal tissues via the directed differentiation of pluripotent stem cells. Methods for differentiation are based on knowledge of years of studying embryonic development of the esophagus in vertebrate animal models. Esophageal tissues derived from human pluripotent stem cells have been used to study both development and diseases affecting the esophagus. Here, we provide a detailed protocol for the directed differentiation of human pluripotent stem cells into human esophageal organoids and organotypic raft cultures, that are highly similar, morphologically and transcriptionally, to the human esophagus epithelium. We discuss limitations of the current esophageal models and the importance of engineering more complex tissue models with muscle and enteric nerves. Moving forward, these models might be utilized for the development of personalized treatments, as well as other therapeutic solutions.
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