Evidence map›Paper›PMID 32586376›Full record

ArticleStem cell research & therapy2020

Shrimp miR-965 induced the human melanoma stem-like cell apoptosis and inhibited their stemness by disrupting the MCL-1-ER stress-XBP1 feedback loop in a cross-species manner.

Wenlin Wu, Chenxi Xu, Xiaobo Zhang, An Yu, Le Shu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell research & therapy, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.0field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 14 citations in OpenAlex.

  1. Article
  2. Article
  3. Shrimp miR-965 transfers tumoricidal mitochondria.Biological procedures online · 2022
    Article
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  5. Review
  6. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 2 countries.

Wenlin WuCollege of Oceanology and Food Science, Quanzhou Normal University, Quanzhou, 36200, Fujian Province, People's Republic of China.
Chenxi XuCollege of Life Science, Zhejiang University, Hangzhou, 310058, Zhejiang Province, People's Republic of China.
Xiaobo ZhangCollege of Life Science, Zhejiang University, Hangzhou, 310058, Zhejiang Province, People's Republic of China.
An YuHuffington Centre on Aging, Baylor College of Medicine, Houston, TX, 77030, USA.
Le ShuCollege of Life Science, Zhejiang University, Hangzhou, 310058, Zhejiang Province, People's Republic of China. leshu@zju.edu.cn.
Zhejiang University · CNBaylor College of Medicine · USQuanzhou Normal University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundMelanoma is a type of aggressive skin cancer with a poor survival rate. The resistance to conventional therapy of this disease is, at least in part, attributed to its cancer stem cell population. However, the mechanism of survival and stemness maintenance of cancer stem cells remains to be investigated.

methodsTumorsphere formation assay was used to study the stem-like property of melanoma stem-like cells (MSLC). Chromatin immunoprecipitation (ChIP), promoter luciferase reporter assay were included for exploring the role of MCL-1 in MSLC and electrophoretic mobility shift assay were used to evaluate the interaction between shrimp miR-965 and human Ago2 protein. Melanoma xenograft nude mice were used to study the inhibition of tumor development.

resultsIn the present study, our results showed that myeloid cell leukemia sequence 1 (MCL-1) knocking down induced ER stress and apoptosis, and the expression reduction of stemness associated genes in MSLC, which implied a significant role of MCL-1 in MSLC. Further study indicated that ER stress agonist (tunicamycin) treatment in MSLC results in the translocation of XBP1, an ER stress sensor, into the nucleus to induce MCL-1 expression through direct binding to the - 313- to - 308-bp region of MCL-1 promoter. In addition, we found that a shrimp-derived miRNA (shrimp miR-965) could interact with the human Ago2 protein and suppressed the human MCL-1 expression by binding to the 3' UTR of MCL-1 mRNA, thereby inhibiting the MSLC proliferation and stemness in vitro and in vivo in a cross-species manner.

conclusionIn conclusion, we identified an important role of MCL-1-ER stress-XBP1 feedback loop in the stemness and survival maintenance of MSLC, and shrimp miR-965, a natural food derived miRNA, could regulate MSLC stemness and survival by targeting MCL-1 and disrupting the balance of MCL-1-ER stress-XBP1 feedback loop. In conclusion, this study indicated an important mechanism of the regulation of MSLC stemness and survival, otherwise it also demonstrated the significance of cross-species-derived miRNA as promising natural drugs in melanoma therapy.

Indexed as

MelanomaMicroRNAsAnimalsApoptosisCell Line, TumorFeedbackGene Expression Regulation, NeoplasticHumansMiceMice, NudeMyeloid Cell Leukemia Sequence 1 ProteinX-Box Binding Protein 1MicroRNAsMyeloid Cell Leukemia Sequence 1 ProteinX-Box Binding Protein 1XBP1 protein, humanCross-species gene expression regulationMelanoma stem-like cellsMyeloid cell leukemia sequence 1 (MCL-1)Shrimp miR-965X-box-binding protein 1 (XBP1)

Identifiers

PMID32586376
PMCPMC7318764
OpenAlexW3037853785

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.