ArticleThe New phytologist2020
Accumulation of the RNA polymerase subunit RpoB depends on RNA editing by OsPPR16 and affects chloroplast development during early leaf development in rice.
Article in The New phytologist, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 57 citations in OpenAlex.
- From Ethnobotany to Pharmacognosy: A Review ofPlants (Basel, Switzerland) · 2026Review
- Anatomy and the chloroplast genome of Bischofia plants reveal important phylogenetic relationship and the genetic diversity in phyllanthaceae.Scientific reports · 2026Article
- Article
- Genome-wide identification and dynamic transcriptome profiling of the DYW-type PPR family across greening of chlorotic leaves in pear (Frontiers in plant science · 2026Article
- SlPPR138-mediated RNA editing ofHorticulture research · 2025Article
- Chloroplast-Localized Protein, OsAL7, with Two Elongation Factor Thermostable Domains Is Essential for Normal Chloroplast Development and Seedling Longevity inPlants (Basel, Switzerland) · 2025Article
- Weighted gene co-expression network analysis - based selection of hub genes related to phenolic and volatile compounds and seed coat color in sorghum.BMC plant biology · 2025Article
- Barley young leaf chlorina, a putative pentatricopeptide repeat gene, is essential for chloroplast development in young leaves.Plant molecular biology · 2025Article
- Article
- The chloroplast pentatricopeptide repeat protein RCN22 regulates tiller number in rice by affecting sugar levels via the TB1-RCN22-RbcL module.Plant communications · 2024Article
- GhCTSF1, a short PPR protein with a conserved role in chloroplast development and photosynthesis, participates in intron splicing of rpoC1 and ycf3-2 transcripts in cotton.Plant communications · 2024Article
- Article
- Chloroplast C-to-U editing, regulated by a PPR protein BoYgl-2, is important for chlorophyll biosynthesis in cabbage.Horticulture research · 2024Article
- PPR proteins in plants: roles, mechanisms, and prospects for rice research.Frontiers in plant science · 2024Review
- Integrated Cytological, Physiological, and Transcriptome Analyses Provide Insight into the Albino Phenotype of Chinese Plum (International journal of molecular sciences · 2023Article
- RNA Editing in Chloroplast: Advancements and Opportunities.Current issues in molecular biology · 2022Review
- A chloroplast-localized pentatricopeptide repeat protein involved in RNA editing and splicing and its effects on chloroplast development in rice.BMC plant biology · 2022Article
- Fujian cytoplasmic male sterility and the fertility restorer geneProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Chloroplastic pentatricopeptide repeat proteins (PPR) in albino plantlets of Agave angustifolia Haw. reveal unexpected behavior.BMC plant biology · 2022Article
- Review
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Authors and funding
16 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plastid-encoded genes are coordinately transcribed by the nucleus-encoded RNA polymerase (NEP) and the plastid-encoded RNA polymerase (PEP). Resulting primary transcripts are frequently subject to RNA editing by cytidine-to-uridine conversions at specific sites. The physiological role of many editing events is largely unknown. Here, we have used the CRISPR/Cas9 technique in rice to knock out a member of the PLS-DYW subfamily of pentatricopeptide repeat (PPR) proteins. We found that OsPPR16 is responsible for a single editing event at position 545 in the chloroplast rpoB messenger RNA (mRNA), resulting in an amino acid change from serine to leucine in the β-subunit of the PEP. In striking contrast to loss-of-function mutations of the putative orthologue in Arabidopsis, which were reported to have no visible phenotype, knockout of OsPPR16 leads to impaired accumulation of RpoB, reduced expression of PEP-dependent genes, and a pale phenotype during early plant development. Thus, by editing the rpoB mRNA, OsPPR16 is required for faithful plastid transcription, which in turn is required for Chl synthesis and efficient chloroplast development. Our results provide new insights into the interconnection of the finely tuned regulatory mechanisms that operate at the transcriptional and post-transcriptional levels of plastid gene expression.
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