Evidence map›Paper›PMID 32583269›Full record

ArticleJournal of cell communication and signaling2020

Phosphoinositide-specific phospholipase C isoforms are conveyed by osteosarcoma-derived extracellular vesicles.

Enrica Urciuoli, Martina Leopizzi, Valeria Di Maio, Stefania Petrini, Valentina D'Oria, Ezio Giorda, Marco Scarsella, Carlo Della Rocca, Vincenza Rita Lo Vasco, Barbara Peruzzi

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of cell communication and signaling, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 46% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 6 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Enrica UrciuoliMultifactorial Disease and Complex Phenotype Research Area, Research Centre, Bambino Gesù Children's Hospital, Rome, Italy.ORCID https://orcid.org/0000-0002-9746-1668
Martina LeopizziDepartment of Medico-Surgical Sciences and Biotechnology, Polo Pontino, Sapienza University, Latina, Rome, Italy.ORCID https://orcid.org/0000-0002-9258-4191
Valeria Di MaioDepartment of Medico-Surgical Sciences and Biotechnology, Polo Pontino, Sapienza University, Latina, Rome, Italy.ORCID https://orcid.org/0000-0002-7851-0989
Stefania PetriniConfocal Microscopy Core Facility, Research Centre, Bambino Gesù Children's Hospital, Rome, Italy.ORCID https://orcid.org/0000-0002-3249-0003
Valentina D'OriaConfocal Microscopy Core Facility, Research Centre, Bambino Gesù Children's Hospital, Rome, Italy.ORCID https://orcid.org/0000-0001-5963-0734
Ezio GiordaFlow Cytometry Core Facility, Research Centre, Bambino Gesù Children's Hospital, Rome, Italy.ORCID https://orcid.org/0000-0002-5875-832X
Marco ScarsellaFlow Cytometry Core Facility, Research Centre, Bambino Gesù Children's Hospital, Rome, Italy.ORCID https://orcid.org/0000-0002-3386-9664
Carlo Della RoccaDepartment of Medico-Surgical Sciences and Biotechnology, Polo Pontino, Sapienza University, Latina, Rome, Italy.ORCID https://orcid.org/0000-0002-7176-5217
Vincenza Rita Lo VascoDepartment of Biomedical, Metabolic and Neural Sciences, Section of Human Morphology, University of Modena and Reggio Emilia, Modena, Italy.ORCID https://orcid.org/0000-0001-7584-6199
Barbara PeruzziMultifactorial Disease and Complex Phenotype Research Area, Research Centre, Bambino Gesù Children's Hospital, Rome, Italy. barbara.peruzzi@opbg.net.ORCID https://orcid.org/0000-0003-1820-5816
Bambino Gesù Children's Hospital · ITUniversity of Modena and Reggio Emilia · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cancer cells are able to release high amounts of extracellular vesicles, thereby conditioning the normal cells in the surrounding tissue and/or in distant target organs. In the context of bone cancers, previous studies suggested that osteosarcoma cancer cells produce transforming extracellular vesicles able to induce a tumour-like phenotype in normal recipient cells. Indeed, phosphoinositide-specific phospholipase C (PI-PLC) enzymes are differentially expressed in osteosarcoma cell lines with increasing aggressiveness, thus providing helpful insights to better define their role and functions in this bone tumour. By confocal microscopy analysis, we demonstrated that osteosarcoma-derived extracellular vesicles convey all the assessed PI-PLC isoforms, and that they localize into cell membrane bubble-like structures, resembling extracellular vesicles about to be released, as conveyed and/or membrane protein. Cytofluorimetric analysis confirmed the presence of PI-PLC isoforms in the extracellular vesicles collected from conditioned media of osteosarcoma cells. These findings suggest the feasibility to use circulating extracellular vesicles as biomarkers of osteosarcoma progression and/or the monitoring of this distressing disease.

Indexed as

Confocal microscopeExtracellular vesiclesFACS analysisOsteosarcomaPI-PLC

Identifiers

PMID32583269
PMCPMC7642127
OpenAlexW3038058377

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.