Evidence map›Paper›PMID 32582961›Full record

ArticleMolecular medicine reports2020

Anti‑chondrocyte apoptosis effect of genistein in treating inflammation‑induced osteoarthritis.

Yang Zou, Qiming Liu, Piaoting Guo, Yang Huang, Zhengcong Ye, Jiong Hu

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular medicine reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
1.2field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 24 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
  4. Article
  5. Review
  6. Article
  7. Article
  8. Review
  9. Review
  10. Review
  11. Review
  12. Genistein attenuates LPSZhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences · 2022
    Article
  13. Review
  14. Review
  15. Article
  16. Review
  17. Therapeutic Single Compounds for Osteoarthritis Treatment.Pharmaceuticals (Basel, Switzerland) · 2021
    Review
  18. Article
  19. Experimental Therapeutics for the Treatment of Osteoarthritis.Journal of experimental pharmacology · 2021
    Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Yang Zou *Department of Orthopedics, The Second Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310005, P.R. China.
Qiming Liu *Department of Orthopedics Surgery, Fuyang Orthopedics and Traumatology Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 311400, P.R. China.
Piaoting GuoDepartment of General Medicine, The Second Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310005, P.R. China.
Yang HuangDepartment of Orthopedics, Municipal Hospital Affiliated to Medical School of Taizhou University, Taizhou, Zhejiang 318000, P.R. China.
Zhengcong YeDepartment of Orthopedics, Xiaoshan Traditional Chinese Medicine Hospital, Hangzhou, Zhejiang 311201, P.R. China.
Jiong HuDepartment of Orthopedics, The Second Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310005, P.R. China.
Zhejiang Chinese Medical University · CNTaizhou University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Osteoarthritis (OA) is a chronic disease that is mainly characterized by chondrocyte degeneration. Inflammatory mediators participate in the development of OA, leading to chondrocyte apoptosis and destruction of the cartilage. Genistein is the major active component of isoflavone, with a chemical composition and a biological effect that is similar to that of estrogens, which prevents the degradation of cartilage; however, its underlying mechanisms of action remain unknown. The aim of the present study was to investigate the anti‑apoptotic effects of genistein on chondrocytes for the treatment of inflammation‑induced OA. Interleukin (IL)‑1β was used to establish a chondrocyte OA model. After treatment with different concentrations of genistein, western blotting identified that expression levels of collagen II and aggrecan were increased in a concentration‑dependent manner, while caspase 3 expression gradually decreased after genistein application. Moreover, flow cytometry and ELISA results demonstrated that genistein could decrease chondrocyte apoptosis and reduce the levels of tumor necrosis factor (TNF)‑α in a dose‑dependent manner. Furthermore, the in vitro data were evaluated in an OA rat model. Genistein increased the collagen and acid glycosaminoglycan content, as well as decreased the levels of TNF‑α and IL‑1β. Genistein also promoted the expression levels of collagen II and aggrecan in the articular cartilage, and decreased the expression of caspase 3, thus alleviating cartilage degradation. In conclusion, the results indicated that genistein mediated inflammation and had an anti‑apoptotic role in treating OA. Therefore, genistein may serve as an alternative treatment for OA.

Indexed as

AggrecansAnimalsAnti-Inflammatory Agents, Non-SteroidalCaspase 3Cells, CulturedCell SurvivalChondrocytesCollagen Type IIDisease Models, AnimalDose-Response Relationship, DrugGene Expression RegulationGenisteinHumansInterleukin-1betaOsteoarthritisPentobarbitalAggrecansAnti-Inflammatory Agents, Non-SteroidalCASP3 protein, humanCaspase 3Collagen Type IIGenisteinInterleukin-1betaPentobarbitalapoptosiscartilage degenerationchondrocytegenisteininflammation

Identifiers

PMID32582961
PMCPMC7411358
OpenAlexW3036419644

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.