ArticlePlant direct2020
A series of dual-reporter vectors for ratiometric analysis of protein abundance in plants.
Article in Plant direct, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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Who cites it
23 citing papers in PubMed.
- Negative feedback regulation of karrikin signaling inProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Salicylic acid modulates its catabolic enzymes via proteasomal degradation linked to SCF-associated proximity networks.Nature communications · 2026Article
- Compensatory Interplay Between Clarin-1 and Clarin-2 Deafness-Associated Proteins Governs Phenotypic Variability in Hearing.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Molecular insights into volatile organic compound sensing and signaling in plants.The Plant journal : for cell and molecular biology · 2026Review
- Causes and consequences of experimental variation in Nicotiana benthamiana transient expression.Nature communications · 2026Article
- An N-terminal domain specifies developmental control by the SMAX1-LIKE family of transcriptional regulators inProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Genetically Encoded, Noise-Tolerant, Auxin Biosensors in Yeast.ACS synthetic biology · 2024Article
- Optimizing Promoters and Subcellular Localization for Constitutive Transgene Expression in Marchantia polymorpha.Plant & cell physiology · 2024Article
- Improved validation of protein interactions using bicistronic BiFC (Bi2FC).Physiology and molecular biology of plants : an international journal of functional plant biology · 2024Article
- Gene Stacking and Stoichiometric Expression of ER-Targeted Constructs Using "2A" Self-Cleaving Peptides.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Quantitative Estimation of Promoter Activity in Cannabis sativa Using Agroinfiltration-Based Transient Gene Expression.Methods in molecular biology (Clifton, N.J.) · 2024Article
- A quantitative autonomous bioluminescence reporter system with a wide dynamic range for Plant Synthetic Biology.Plant biotechnology journal · 2024Article
- KARRIKIN UP-REGULATED F-BOX 1 (KUF1) imposes negative feedback regulation of karrikin and KAI2 ligand metabolism inProceedings of the National Academy of Sciences of the United States of America · 2022Article
- The strigolactone receptor D14 targets SMAX1 for degradation in response to GR24 treatment and osmotic stress.Plant communications · 2022Article
- Rapid analysis of strigolactone receptor activity in aPlant direct · 2022Article
- Strigo-D2-a bio-sensor for monitoring spatio-temporal strigolactone signaling patterns in intact plants.Plant physiology · 2022Article
- Hairy CRISPR: Genome Editing in Plants Using Hairy Root Transformation.Plants (Basel, Switzerland) · 2021Review
- Fluorescent biosensors illuminating plant hormone research.Plant physiology · 2021Review
- The mechanism of host-induced germination in root parasitic plants.Plant physiology · 2021Review
- Biosensors: A Sneak Peek into Plant Cell's Immunity.Life (Basel, Switzerland) · 2021Review
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ratiometric reporter systems enable comparisons of the abundance of a protein of interest, or "target," relative to a reference protein. Both proteins are encoded on a single transcript but are separated during translation. This arrangement bypasses the potential for discordant expression that can arise when the target and reference proteins are encoded by separate genes. We generated a set of 18 Gateway-compatible vectors termed pRATIO that combine a variety of promoters, fluorescent, and bioluminescent reporters, and 2A "self-cleaving" peptides. These constructs are easily modified to produce additional combinations or introduce new reporter proteins. We found that mScarlet-I provides the best signal-to-noise ratio among several fluorescent reporter proteins during transient expression experiments in
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.