Determining the Effect of Amino Acids on the Allergenic Activity of Pollen Extracts.

Nazila Ariaee, Mojtaba Sankian, Abdolreza Varasteh, Mitra Hosseinpour, Farahzad Jabbari

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Article in Reports of biochemistry & molecular biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

0.1field-weighted citation impact, top 52% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

Introducing a Stabilizer Formulation for Allergenic Mold Extracts.Reports of biochemistry & molecular biology · 2020
Article

4 · The record

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5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Nazila AriaeeAllergy Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.

Mojtaba SankianImmunology Research Center, Medical School, Mashhad University of Medical Sciences, Mashhad, Iran.

Abdolreza VarastehAllergy Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.

Mitra HosseinpourShahid Beheshti University of Medical Sciences, 15 Khordad Hospital, Tehran, Iran.

Farahzad JabbariAllergy Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.

Mashhad University of Medical Sciences · IRShahid Beheshti University of Medical Sciences · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe diagnosis and treatment of allergic diseases require high quality pollen allergen extracts for reliable test results and effective treatments. The quality of the pollen allergen extracts is influenced by pharmacologically inert ingredients, such as stabilizers which are added to prevent the degradation of the allergenic activity. This study was conducted to develop a stabilizer formulation in order to protect the allergenic activity of the pollen's extracts.

methodsPine and orchard grass pollen allergen extracts were incubated for 40 days at 37 °C. The effects of chemicals were examined via inhibition ELISA on days 7, 14, 21, 28, and 40 to evaluate the ability of the pollen allergen extracts to inhibit specific IgE in the sera of sensitized patients.

resultsOur findings showed that the pine pollen and orchard grass allergen extracts treated with Lys/Glu had the best stabilizing effect resulting in a 97% IgE inhibition following the 40 days of incubation. In the non-treatment group, the IgE inhibition decreased to 23% at the end of the 40 days. The orchard grass pollen allergen extracts receiving no treatment decreased to 12% IgE inhibition following the 40-day incubation.

conclusionAmino acids are able to act as an effective stabilizer for pollen allergen extracts and prevent the degradation of their activity over time. Particularly applying Lys/ Glu in pollen allergenic extracts can protect allergenic activity and potency of the pollen extracts to inhibit specific IgE in human sera.

Indexed as

Amino acidsPollenSkin Prick TestStabilizing

Identifiers

PMID32582797

PMCPMC7275837

OpenAlexW3030484021

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.