Evidence map›Paper›PMID 32582794›Full record

ArticleReports of biochemistry & molecular biology2020

Swapping of The N-Terminal Domain of Human Topoisomerase 1B with the Corresponding

Jagadish Babu Dasari, Bini Chhetri Soren, Alessio Ottaviani, Cinzia Tesauro, Simona Marino, Beatrice Messina, Paola Fiorani

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Article in Reports of biochemistry & molecular biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

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5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Jagadish Babu DasariDepartment of Biology, University of Rome Tor Vergata, Via Della Ricerca Scientifica 1, 00133 Rome, Italy.
Bini Chhetri SorenDepartment of Biology, University of Rome Tor Vergata, Via Della Ricerca Scientifica 1, 00133 Rome, Italy.
Alessio OttavianiDepartment of Biology, University of Rome Tor Vergata, Via Della Ricerca Scientifica 1, 00133 Rome, Italy.
Cinzia TesauroDepartment of Biology, University of Rome Tor Vergata, Via Della Ricerca Scientifica 1, 00133 Rome, Italy.
Simona MarinoDepartment of Biology, University of Rome Tor Vergata, Via Della Ricerca Scientifica 1, 00133 Rome, Italy.
Beatrice MessinaDepartment of Biology, University of Rome Tor Vergata, Via Della Ricerca Scientifica 1, 00133 Rome, Italy.
Paola FioraniDepartment of Biology, University of Rome Tor Vergata, Via Della Ricerca Scientifica 1, 00133 Rome, Italy.
University of Rome Tor Vergata · ITIstituto di Farmacologia Traslazionale · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundDNA topoisomerases 1B are a class of ubiquitous enzyme that solves the topological problems associated with biological processes such as replication, transcription and recombination. Numerous sequence alignment of topoisomerase 1B from different species shows that the lengths of different domains as well as their amino acids sequences are quite different. In the present study a hybrid enzyme, generated by swapping the N-terminal of

methodsThe chimeric enzyme was generated using different sets of PCR. The

resultsThe chimeric enzyme displayed slower relaxation activity, cleavage and re-ligation kinetics strongly perturbed when compared to the human enzyme.

conclusionThese results indicate that the N-terminal domain has a crucial role in modulating topoisomerase activity in different species.

Indexed as

N-terminal domainPlasmodium falciparum topoisomerase 1BTopoisomerase 1B

Identifiers

PMID32582794
PMCPMC7275839
OpenAlexW3030592857

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