ArticleClinical proteomics2020
Identification of pathogens from native urine samples by MALDI-TOF/TOF tandem mass spectrometry.
Article in Clinical proteomics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 22 citations in OpenAlex.
- An Overview of the Association of the Urinary Tract Microbiome with Various Diseases and Implications for Therapeutics.Mini reviews in medicinal chemistry · 2025Review
- Detection of methicillin resistance of Staphylococcus aureus in vitreous humor using MALDI-TOF MS and Fc-MBL@FeScientific reports · 2024Article
- Accelerated Bacterial Identification with MALDI-TOF MS Leads to Fewer Diagnostic Tests and Cost Savings.Antibiotics (Basel, Switzerland) · 2024Article
- Comprehensive insights into UTIs: from pathophysiology to precision diagnosis and management.Frontiers in cellular and infection microbiology · 2024Review
- Seed protein biotyping in Amaranthus species: a tool for rapid identification of weedy amaranths of concern.Plant methods · 2023Article
- Clinical Impact of Rapid Bacterial Microbiological Identification with the MALDI-TOF MS.Antibiotics (Basel, Switzerland) · 2023Article
- Current Viewpoint on Female Urogenital Microbiome-The Cause or the Consequence?Microorganisms · 2023Review
- Applications of MALDI-MS/MS-Based Proteomics in Biomedical Research.Molecules (Basel, Switzerland) · 2022Review
- Proteomics approaches: A review regarding an importance of proteome analyses in understanding the pathogens and diseases.Frontiers in veterinary science · 2022Review
- CD5L as an Extracellular Vesicle-Derived Biomarker for Liquid Biopsy of Lung Cancer.Diagnostics (Basel, Switzerland) · 2021Article
- Modeling of Urinary Microbiota Associated With Cystitis.Frontiers in cellular and infection microbiology · 2021Article
- New Microbiological Techniques for the Diagnosis of Bacterial Infections and Sepsis in ICU Including Point of Care.Current infectious disease reports · 2021Review
- MALDI-TOF Mass Spectroscopy Applications in Clinical Microbiology.Advances in pharmacological and pharmaceutical sciences · 2021Review
- Pathobionts: mechanisms of survival, expansion, and interaction with host with a focus onGut microbesArticle
Corrections and comments
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Authors and funding
11 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundReliable high-throughput microbial pathogen identification in human urine samples is crucial for patients with cystitis symptoms. Currently employed methods are time-consuming and could lead to unnecessary or inadequate antibiotic treatment. Purpose of this study was to assess the potential of mass spectrometry for uropathogen identification from a native urine sample.
methodsIn total, 16 urine samples having more than 10
resultsHere we present advantages and disadvantages of bottom-up proteomics, using MALDI-TOF/TOF tandem mass spectrometry, for culture-independent identification of uropathogens (e.g. directly from urine samples). The direct approach provided reliable identification of bacteria at the genus level in monobacterial samples. Taxonomic identifications obtained by proteomics were compared both to standard urine culture test used in clinics and genomic test based on 16S rRNA sequencing.
conclusionsOur findings indicate that mass spectrometry has great potential as a reliable high-throughput tool for microbial pathogen identification in human urine samples. In this case, the MALDI-TOF/TOF, was used as an analytical tool for the determination of bacteria in urine samples, and the results obtained emphasize high importance of storage conditions and sample preparation method impacting reliability of MS2 data analysis. The proposed method is simple enough to be utilized in existing clinical settings and is highly suitable for suspected single organism infectious etiologies. Further research is required in order to identify pathogens in polymicrobial urine samples.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.