ArticleProceedings of the National Academy of Sciences of the United States of America2020
Three types of HLA-G+ extravillous trophoblasts that have distinct immune regulatory properties.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 68 citations in OpenAlex.
- Gene regulation at the early human maternal-fetal interface.Nature reviews. Genetics · 2026Review
- Purification of HLA-G+ Extravillous Trophoblasts from Human Term Placental Tissues for Phenotyping and Functional Analysis.Journal of visualized experiments : JoVE · 2026Article
- Unlocking the promise of innate biology through the HLA-G/ILT2/ILT4 pathway.Journal for immunotherapy of cancer · 2026Review
- Decidual CD4 Effector Memory and Resident Memory T Cells Respond to Cord Blood Allo-Antigens.American journal of reproductive immunology (New York, N.Y. : 1989) · 2026Article
- Immunomodulatory effect of mesenchymal stromal cell overexpressing HLA-G1 in cell-based therapy for myocardial infarction.Stem cell research & therapy · 2026Article
- Common chromosomal aneuploidy testing using non-invasive trophoblast retrieval and isolation from the cervix (TRIC) in pregnant women.Archives of gynecology and obstetrics · 2025Article
- Defining cellular diversity at the swine maternal-fetal interface using spatial transcriptomics and organoids.PLoS biology · 2025Article
- First trimester extravillous trophoblast secretes HLA class I molecules via small extracellular vesicles.Placenta · 2025Article
- Research design and tissue collection considerations for investigation of placenta accreta spectrum.Placenta · 2025Article
- Development of apical out trophoblast stem cell derived organoids to model early human pregnancy.iScience · 2025Article
- Dynamic roles of ILC3 in endometrial repair and regeneration.Discovery immunology · 2025Article
- Establishment and comparison of human term placenta-derived trophoblast cells†.Biology of reproduction · 2024Article
- The Effect of the cAMP Signaling Pathway on HTR8/SV-Neo Cell Line Proliferation, Invasion, and Migration After Treatment with Forskolin.Reproductive sciences (Thousand Oaks, Calif.) · 2024Article
- HLA-G Expression/Secretion and T-Cell Cytotoxicity in Missed Abortion in Comparison to Normal Pregnancy.International journal of molecular sciences · 2024Article
- Combined maternal KIR2DL4 and fetal HLA-G polymorphisms were associated with preeclampsia in a Han Chinese population.Frontiers in genetics · 2024Article
- Placenta: an old organ with new functions.Frontiers in immunology · 2024Review
- Antigen-specific decidual CD8+ T cells include distinct effector memory and tissue-resident memory cells.JCI insight · 2023Article
- Article
- The Molecular Mechanisms of HLA-G Regulatory Function on Immune Cells during Early Pregnancy.Biomolecules · 2023Review
- A Comparative Review of Pregnancy and Cancer and Their Association with Endoplasmic Reticulum Aminopeptidase 1 and 2.International journal of molecular sciences · 2023Review
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
During pregnancy, invading HLA-G+ extravillous trophoblasts (EVT) play a key role in placental development, uterine spiral artery remodeling, and prevention of detrimental maternal immune responses to placental and fetal antigens. Failures of these processes are suggested to play a role in the development of pregnancy complications, but very little is known about the underlying mechanisms. Here we present validated methods to purify and culture primary HLA-G+ EVT from the placental disk and chorionic membrane from healthy term pregnancy. Characterization of HLA-G+ EVT from term pregnancy compared to first trimester revealed their unique phenotypes, gene expression profiles, and differing capacities to increase regulatory T cells (Treg) during coculture assays, features that cannot be captured by using surrogate cell lines or animal models. Furthermore, clinical variables including gestational age and fetal sex significantly influenced EVT biology and function. These methods and approaches form a solid basis for further investigation of the role of HLA-G+ EVT in the development of detrimental placental inflammatory responses associated with pregnancy complications, including spontaneous preterm delivery and preeclampsia.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.