ArticleInternational journal of molecular sciences2020
Effect of Oocyte Quality Assessed by Brilliant Cresyl Blue (BCB) Staining on Cumulus Cell Expansion and Sonic Hedgehog Signaling in Porcine during In Vitro Maturation.
Article in International journal of molecular sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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Who cites it
9 citing papers in PubMed, 24 citations in OpenAlex.
- Single-Cell RNA Sequencing Reveals Molecular Differences Underlying Meiotic Competence of Brilliant Cresyl Blue (BCB) Selected Alpaca Oocytes.Animals : an open access journal from MDPI · 2026Article
- Multi-Omics Reveal the Metabolic Changes in Cumulus Cells During Aging.Cell proliferation · 2025Article
- Article
- Restoration of Developmental Competence in Low-Quality Porcine Cumulus-Oocyte Complexes through the Supplementation of Sonic Hedgehog Protein during In Vitro Maturation.Animals : an open access journal from MDPI · 2023Article
- Anethole improves the developmental competence of porcine embryos by reducing oxidative stress via the sonic hedgehog signaling pathway.Journal of animal science and biotechnology · 2023Article
- Transient suppression of Wnt signaling in poor-quality buffalo oocytes improves their developmental competence.Frontiers in veterinary science · 2023Article
- Tight gene co-expression in BCB positive cattle oocytes and their surrounding cumulus cells.Reproductive biology and endocrinology : RB&E · 2022Article
- Luteolin Orchestrates Porcine Oocyte Meiotic Progression by Maintaining Organelle Dynamics Under Oxidative Stress.Frontiers in cell and developmental biology · 2021Article
- Proteomic Exploration of Porcine Oocytes During Meiotic MaturationFrontiers in veterinary science · 2021Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
Brilliant cresyl blue (BCB) staining is used to select developmentally competent cumulus-oocyte complexes (COCs) for in vitro maturation (IVM). However, limited attention has been paid to what drives the higher developmental competence of BCB+ COCs. Sonic hedgehog signaling (SHH) is an important signaling pathway for ovarian follicular development and oocyte maturation. Therefore, this study investigated the effect of oocyte quality assessed by BCB staining on cumulus cell expansion, oocyte nuclear maturation, subsequent embryo development, apoptosis levels, and SHH signaling protein expression, in porcine COCs. After IVM, BCB+ COCs exhibited a significantly higher proportion of complete cumulus cell expansion and metaphase II rate in oocytes than BCB- COCs. After in vitro fertilization, the BCB+ group showed a significantly higher monospermy rate, fertilization efficiency, percentage of cleavage and blastocyst formation, with a higher total cell number and a lower apoptosis in blastocysts as compared with the BCB- group. Furthermore, significantly lower apoptosis levels and a higher expression of SHH-signaling proteins in COCs were observed, before and after IVM. In conclusion, high-quality oocytes had a greater potential to expand their surrounding cumulus cells with active SHH signaling and a lower apoptosis. This could provide COCs with a proper environment for maturation, thereby leading to a better subsequent embryo development.
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