Evidence map›Paper›PMID 32580265›Full record

ArticleJournal of Korean Neurosurgical Society2020

Survival Association and Cell Cycle Effects of B7H3 in Neuroblastoma.

Haibo Zhang, Jinsen Zhang, Chunjie Li, Hao Xu, Rui Dong, Clark C Chen, Wei Hua

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of Korean Neurosurgical Society, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
1.3field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 24 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 2 countries.

Haibo ZhangDepartment of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, P.R. China.
Jinsen ZhangDepartment of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, P.R. China.
Chunjie LiInstitutes of Biomedical Sciences, Fudan University, Shanghai, P.R. China.
Hao XuDepartment of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, P.R. China.
Rui DongDepartment of Pediatric Surgery, Children's Hospital of Fudan University, Shanghai, China.
Clark C ChenDepartment of Neurosurgery, University of Minnesota, Minneapolis, MN, USA.
Wei HuaDepartment of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, P.R. China.
Fudan University · CNChildren's Hospital of Fudan University · CNUniversity of Minnesota · US

Funding

Targeting Mechanisms of Acquired Temozolomide Resistance in GlioblastomaR01NS097649 · NINDS · UNIVERSITY OF MINNESOTA · PI CHEN, CLARK CHIN-CHUNG · 2016 to 2021
$1.7M
Doris Duke Charitable Foundation 1RO1NS097649-01National Natural Science Foundation of China 81572483National Natural Science Foundation of China 81611130223Natural Science Foundation and Major Basic Research Program of Shanghai 16JC1420100NINDS NIH HHS R01 NS097649Program of International Science & Technology Cooperation of China 2014DFA31470Shanghai Committee of Science and Technology 15441904500Sontag Foundation BWF 1006774.01
6 · The paper itself

Abstract

objectiveThe function of B7H3, a member of the B7 family of proteins, in neuroblastoma (NB) remains poorly characterized. Here we examine the expression pattern of B7H3 in clinical NB specimens and characterize the phenotype of B7H3 knock-down in NB cell line.

methodsImmunohistochemical (IHC) staining was carried out to assess the expression of B7H3 in clinical NB specimens. Survival association was analyzed using five Gene Expression Omnibus (GEO) datasets (GSE85047, GSE45480, GSE62564, GSE16476, GSE49710). Clonogenic survival and flow cytometry were performed after B7H3 knockdown to assess the cellular proliferation and cell survival in vitro. Impact of B7H3 silencing on NB growth was examined in vivo using the SH-SY5Y xenograft model.

resultsOn IHC staining, B7H3 was widely expressed in clinical NB specimens. Analysis of the transcriptional profiles of five GEO datasets clinically annotated NB specimens revealed that decreased B7H3 expression was associated with improved overall survival. B7H3 knockdown suppressed the proliferation of the SH-SY5Y NB model in vitro and in vivo. Cell cycle analysis revealed that B7H3 silencing induced G1/S arrest. This arrest was associated with the suppression of E2F1 expression and induction of Rb expression.

conclusionOur results demonstrate that B7H3 expression correlate with clinical survival in NB patients. Preliminary studies suggest that B7H3 may mediate the G1/S transition.

Indexed as

B7H3Cell cycleNeuroblastomaPrognosisProliferation

Identifiers

PMID32580265
PMCPMC7671786
OpenAlexW3037406955

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.