ArticleMolecular biology of the cell2020
Initiation and disassembly of filopodia tip complexes containing VASP and lamellipodin.
Article in Molecular biology of the cell, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 52 citations in OpenAlex.
- Membrane Kymograph Generator: a cross-platform GUI software for automated generation and analysis of kymographs along dynamic cell boundaries.Bioinformatics (Oxford, England) · 2026Article
- A balance between nucleating and elongating actin filaments controls deformation of protein condensates.Science advances · 2026Article
- Mechanochemical feedback between confinement and actin crosslinking drives the shape dynamics of liquid-like droplets.Nature communications · 2026Article
- Membrane Kymograph Generator: A cross-platform GUI software for automated generation and analysis of kymographs along dynamic cell boundaries.bioRxiv : the preprint server for biology · 2026Article
- Roles of cytoskeleton in metastasis: from its mechanism to therapeutic strategies.Experimental & molecular medicine · 2026Review
- Liquid-like condensates that bind actin promote assembly and bundling of actin filaments.Developmental cell · 2025Article
- Membrane-associated polymerases deliver most of the actin subunits to a lamellipodial network.bioRxiv : the preprint server for biology · 2025Article
- Spatiotemporal coordination of actin regulators generates invasive protrusions in cell-cell fusion.Nature cell biology · 2024Article
- Fascin-1 Promotes Cell Metastasis through Epithelial-Mesenchymal Transition in Canine Mammary Tumor Cell Lines.Veterinary sciences · 2024Article
- Liquid-like condensates that bind actin drive filament polymerization and bundling.bioRxiv : the preprint server for biology · 2024Article
- Archaeal actins and the origin of a multi-functional cytoskeleton.Journal of bacteriology · 2024Review
- Filopodial protrusion driven by density-dependent Ena-TOCA-1 interactions.Journal of cell science · 2024Article
- Liquid-like condensates mediate competition between actin branching and bundling.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Reconstitution of the transition from a lamellipodia- to filopodia-like actin network with purified proteins.European journal of cell biology · 2023Article
- Chiral growth of adherent filopodia.Biophysical journal · 2023Article
- Liquid-like condensates mediate competition between actin branching and bundling.bioRxiv : the preprint server for biology · 2023Article
- Liquid-like VASP condensates drive actin polymerization and dynamic bundling.Nature physics · 2023Article
- Ena/VASP clustering at microspike tips involves lamellipodin but not I-BAR proteins, and absolutely requires unconventional myosin-X.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Biochemical and mechanical regulation of actin dynamics.Nature reviews. Molecular cell biology · 2022Review
- PtdIns(3,4)P2, Lamellipodin, and VASP coordinate actin dynamics during phagocytosis in macrophages.The Journal of cell biology · 2022Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
The shapes of many eukaryotic cells depends on the actin cytoskeleton, and changes in actin assembly dynamics underlie many changes in cell shape. Ena/VASP-family actin polymerases, for example, modulate cell shape by accelerating actin filament assembly locally and slowing filament capping. When concentrated into discrete foci at the leading edge, VASP promotes filopodia assembly and forms part of a poorly understood molecular complex that remains associated with growing filopodia tips. Here we identify precursors of this filopodia tip complex in migrating B16F1 cells: small leading-edge clusters of the adaptor protein lamellipodin (Lpd) that subsequently recruit VASP and initiate filopodia formation. Dimerization, membrane association, and VASP binding are all required for lamellipodin to incorporate into filopodia tip complexes, and overexpression of monomeric, membrane--targeted lamellipodin mutants disrupts tip complex assembly. Once formed, tip complexes containing VASP and lamellipodin grow by fusing with each other, but their growth is limited by a size-dependent dynamic instability. Our results demonstrate that assembly and disassembly dynamics of filopodia tip complexes are determined, in part, by a network of multivalent interactions between Ena/VASP proteins, EVH1 ligands, and actin filaments.
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