Evidence map›Paper›PMID 32579048›Full record

ArticleIslets2020

Selective monitoring of insulin secretion after CRISPR interference in intact pancreatic islets despite submaximal infection.

Kaavian Shariati, Zachary Pappalardo, Deeksha G Chopra, Nicholas Yiv, Robin Sheen, Gregory Ku

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Islets, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Kaavian ShariatiDiabetes Center, UCSF , San Francisco, CA, USA.ORCID 0000-0002-0453-4275
Zachary PappalardoDiabetes Center, UCSF , San Francisco, CA, USA.ORCID 0000-0002-7290-520X
Deeksha G ChopraDiabetes Center, UCSF , San Francisco, CA, USA.
Nicholas YivDiabetes Center, UCSF , San Francisco, CA, USA.ORCID 0000-0002-7851-7945
Robin SheenDiabetes Center, UCSF , San Francisco, CA, USA.
Gregory KuDiabetes Center, UCSF , San Francisco, CA, USA.ORCID 0000-0001-5306-4225
University of California, San Francisco · US

Funding

Research BaseP30DK063720 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI GERMAN, MICHAEL S · 2003 to 2019
$21.8M
The role of Spry2 in beta cell function and the unfolded protein responseR01DK107650 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI KU, GREGORY MICHAEL · 2016 to 2020
$1.8M
The role of mitochondrial fission in beta cell functionR01DK118337 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI KU, GREGORY MICHAEL · 2020 to 2023
$1.5M
Discovering and dissecting new regulators of insulin production in beta cellsK08DK087945 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI KU, GREGORY MICHAEL · 2011 to 2015
$747k
NIDDK NIH HHS K08 DK087945NIDDK NIH HHS P30 DK063720NIDDK NIH HHS R01 DK107650NIDDK NIH HHS R01 DK118337
6 · The paper itself

Abstract

Virus-mediated gene knockdown in intact pancreatic islets is technically challenging due to poor infection of the center of the islet. Because the cells that do not have knockdown have normal insulin secretion, measuring changes in insulin secretion after gene knockdown is challenging. We describe a method to monitor insulin secretion from only the beta cells with knockdown of a gene of interest in intact islets using a single lentivirus containing a guide RNA, a luciferase insulin secretion reporter and a dCas9-KRAB cassette. This method allows rapid and inexpensive monitoring of insulin secretion from only those beta cells with knockdown, circumventing the problem of incomplete islet infection.

Indexed as

CRISPR-Associated Protein 9CRISPR-Cas SystemsGene EditingAnimalsBodily SecretionsGene Knockdown TechniquesInsulinIslets of LangerhansMiceRNA InterferenceCRISPR-Associated Protein 9InsulinCRISPRInsulin secretionisletRNA interference

Identifiers

PMID32579048
PMCPMC7527018
OpenAlexW3037406682

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.