Evidence map›Paper›PMID 32578684›Full record

ArticleMemorias do Instituto Oswaldo Cruz2020

Analysing ambiguities in trypanosomatids taxonomy by barcoding.

Carolina Boucinha, Amanda R Caetano, Helena Lc Santos, Raphael Helaers, Miikka Vikkula, Marta Helena Branquinha, André Luis Souza Dos Santos, Philippe Grellier, Karina Alessandra Morelli, Claudia Masini d'Avila-Levy

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Memorias do Instituto Oswaldo Cruz, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.7field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 6 citations in OpenAlex.

  1. Microorganisms · 2025
    Article
  2. The Haptomonad Stage ofVeterinary sciences · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 5 institutions in 3 countries.

Carolina BoucinhaFundação Oswaldo Cruz-Fiocruz, Instituto Oswaldo Cruz, Laboratório de Estudos Integrados em Protozoologia, Coleção de Protozoários da Fiocruz, Rio de Janeiro, RJ, Brasil.
Amanda R CaetanoFundação Oswaldo Cruz-Fiocruz, Instituto Oswaldo Cruz, Laboratório de Estudos Integrados em Protozoologia, Coleção de Protozoários da Fiocruz, Rio de Janeiro, RJ, Brasil.
Helena Lc SantosFundação Oswaldo Cruz-Fiocruz, Instituto Oswaldo Cruz, Laboratório de Estudos Integrados em Protozoologia, Coleção de Protozoários da Fiocruz, Rio de Janeiro, RJ, Brasil.
Raphael HelaersUniversity of Louvain, de Duve Institute, Laboratory of Human Molecular Genetics, Brussels, Belgium.
Miikka VikkulaUniversity of Louvain, de Duve Institute, Laboratory of Human Molecular Genetics, Brussels, Belgium.
Marta Helena BranquinhaUniversidade Federal do Rio de Janeiro, Instituto de Microbiologia Paulo de Góes, Rio de Janeiro, Brasil.
André Luis Souza Dos SantosUniversidade Federal do Rio de Janeiro, Instituto de Microbiologia Paulo de Góes, Rio de Janeiro, Brasil.
Philippe GrellierMuséum National d'Histoire Naturelle, Unité Molécules de Communication et Adaptation des Microorganisme, Paris, France.
Karina Alessandra MorelliFundação Oswaldo Cruz-Fiocruz, Instituto Oswaldo Cruz, Laboratório de Estudos Integrados em Protozoologia, Coleção de Protozoários da Fiocruz, Rio de Janeiro, RJ, Brasil.ORCID http://orcid.org/0000-0002-8456-9864
Claudia Masini d'Avila-LevyFundação Oswaldo Cruz-Fiocruz, Instituto Oswaldo Cruz, Laboratório de Estudos Integrados em Protozoologia, Coleção de Protozoários da Fiocruz, Rio de Janeiro, RJ, Brasil.ORCID http://orcid.org/0000-0001-8042-4695
de Duve Institute · BEFundação Oswaldo Cruz · BRUniversidade Federal do Rio de Janeiro · BRMuséum national d'Histoire naturelle · FRUniversidade do Estado do Rio de Janeiro · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundBiodiversity screens and phylogenetic studies are dependent on reliable DNA sequences in public databases. Biological collections possess vouchered specimens with a traceable history. Therefore, DNA sequencing of samples available at institutional collections can greatly contribute to taxonomy, and studies on evolution and biodiversity.

methodsWe sequenced part of the glycosomal glyceraldehyde phosphate dehydrogenase (gGAPDH) and the SSU rRNA (V7/V8) genes from 102 trypanosomatid cultures, which are available on request at www.colprot.fiocruz.br.

objectiveThe main objective of this work was to use phylogenetic inferences, using the obtained DNA sequences and those from representatives of all Trypanosomatidae genera, to generate phylogenetic trees that can simplify new isolates screenings.

findingsA DNA sequence is provided for the first time for several isolates, the phylogenetic analysis allowed the classification or reclassification of several specimens, identification of candidates for new genera and species, as well as the taxonomic validation of several deposits. MAIN

conclusionsThis survey aimed at presenting a list of validated species and their associated DNA sequences combined with a short historical overview of each isolate, which can support taxonomic and biodiversity research and promote culture collections.

Indexed as

BiodiversityDNA Barcoding, TaxonomicPhylogenyTrypanosomatina

Identifiers

PMID32578684
PMCPMC7304411
OpenAlexW3036889732

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.