Evidence map›Paper›PMID 32578051›Full record

ArticleHuman cell2020

MiR-96 promotes apoptosis of nucleus pulpous cells by targeting FRS2.

Xiaoxia Yang, Hengping Liu, Qingfeng Zhang, Kan Liu, Dong Yu, Yi Zhang, Zongting Shi

Erratum issuedAbstract read
PubMed Publisher
In one paragraph

Article in Human cell, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.7field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 8 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Review
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  6. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Xiaoxia YangDepartment of Spine, Beijing University of Chinese Medicine Third Affiliated Hospital, No.51 Xiaoguan Street, Outside, Andingmen, Beijing, 100029, China. yangxiaoxia_12@126.com.
Hengping LiuDepartment of Spine, Beijing University of Chinese Medicine Third Affiliated Hospital, No.51 Xiaoguan Street, Outside, Andingmen, Beijing, 100029, China.
Qingfeng ZhangDepartment of Spine, Beijing University of Chinese Medicine Third Affiliated Hospital, No.51 Xiaoguan Street, Outside, Andingmen, Beijing, 100029, China.
Kan LiuDepartment of Spine, Beijing University of Chinese Medicine Third Affiliated Hospital, No.51 Xiaoguan Street, Outside, Andingmen, Beijing, 100029, China.
Dong YuDepartment of Spine, Beijing University of Chinese Medicine Third Affiliated Hospital, No.51 Xiaoguan Street, Outside, Andingmen, Beijing, 100029, China.
Yi ZhangDepartment of Spine, Beijing University of Chinese Medicine Third Affiliated Hospital, No.51 Xiaoguan Street, Outside, Andingmen, Beijing, 100029, China.
Zongting ShiDepartment of Spine, Beijing University of Chinese Medicine Third Affiliated Hospital, No.51 Xiaoguan Street, Outside, Andingmen, Beijing, 100029, China.
Beijing University of Chinese Medicine · CN

Funding

National Natural Science Foundation of China 81772412National Natural Science Foundation of China 81802190Shandong Medical and Health Technology Development Plan Project 2017WS033Youth Fund Project of the Affiliated Hospital of Qingdao University 2017
6 · The paper itself

Abstract

This study aimed to investigate the molecular mechanism by which microRNA (miR)-96 regulates the progression of intervertebral disc degeneration (IDD). The expression of miR-96 in normal intervertebral discs and in IDD was detected by performing reverse transcription-quantitative PCR. CCK8 assay was applied to examine the proliferation of nucleus pulpous (NP) cells and flow cytometry was used to evaluate the cell apoptosis and cell cycle profile. In addition, the immunofluorescence analysis was employed to detect cell proliferation. The expressions of proteins were assessed by western blot analysis. TargetScan and miRDB were used to predict the target genes of miR-96. The results indicated that miR-96 expression was upregulated in IDD compared with normal intervertebral discs. Overexpression of miR-96 could significantly inhibit the proliferation of NP cells via inducing apoptosis and G1 arrest. In addition, fibroblast growth factor receptor substrate 2 (FRS2) was identified as the target of miR-96 and overexpression of FRS2 could revere the effect of miR-96 mimics in NP cells. Therefore, these findings demonstrated that miR-96 plays a critical role during the progression of IDD and miR-96 may serve as a target for the treatment of IDD.

Indexed as

Gene ExpressionAdaptor Proteins, Signal TransducingAdultApoptosisCell ProliferationCells, CulturedDisease ProgressionHumansIntervertebral Disc DegenerationMembrane ProteinsMicroRNAsMiddle AgedNucleus PulposusUp-RegulationAdaptor Proteins, Signal TransducingFRS2 protein, humanMembrane ProteinsMicroRNAsMIRN96 microRNA, humanCell cycleFibroblast growth factor receptor substrate 2Intervertebral disc degenerationmiR-96

Identifiers

PMID32578051
OpenAlexW3036160417

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.