Evidence map›Paper›PMID 32577945›Full record

ArticleMolecular and cellular biochemistry2020

Purification and biochemical characterization of a novel secretory dipeptidyl peptidase IV from porcine serum.

Divya Kumar, Vivek K Hamse, K N Neema, Priya Babu Shubha, D M Chetan, Nanjunda Swamy Shivananju

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Article in Molecular and cellular biochemistry, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 4 institutions in 1 country.

Divya KumarDepartment of Biotechnology, Sri Jayachamarajendra College of Engineering, JSS Science and Technology University, JSS TI Campus, Mysuru, Karnataka, 570006, India.
Vivek K HamseFaculty of Natural Sciences, Adichunchanagiri University, Bellur Cross, B.G. Nagara, Mandya, Karnataka, India.
K N NeemaDepartment of Biotechnology, Sri Jayachamarajendra College of Engineering, JSS Science and Technology University, JSS TI Campus, Mysuru, Karnataka, 570006, India.
Priya Babu ShubhaDepartment of Studies in Chemistry, University of Mysore, Manasagangothri, Mysuru, Karnataka, 570006, India.
D M ChetanDepartment of Biotechnology, NMAM Institute of Technology, Nitte, Karkala Taluk, Udupi, Karnataka, 574110, India.
Nanjunda Swamy ShivananjuDepartment of Biotechnology, Sri Jayachamarajendra College of Engineering, JSS Science and Technology University, JSS TI Campus, Mysuru, Karnataka, 570006, India. nanjundaswamy@sjce.ac.in.ORCID http://orcid.org/0000-0002-5494-9428
JSS Science and Technology University · INJSS Academy of Higher Education and Research · INNational Institute of Technology Karnataka · INUniversity of Mysore · IN

Funding

Department of Science and Technology, Govt of India, Nw Delhi, India DST/INDO/SOUTH AFRICA/P-12/2014Indian Institute of Science (IN) Approval Letter No: 45/33/2012/BMS/TRM
6 · The paper itself

Abstract

Purification of DPP-IV enzyme from porcine serum, is presented in this study for the first time. The high molecular weight DPP-IV from porcine serum was fractioned using Sephadex G-75 gel filtration followed by DEAE Sephadex anion exchange and Sephadex G-100 gel filtration chromatography columns with a final yield of 11.25%. The SDS-PAGE of the purified sample showed a single band of molecular mass nearing 160 kDa. Distinct single band was observed after PAS staining confirmed it to be a glycoprotein. The purified enzyme showed an optimum pH and temperature of 8 and 37 °C, respectively. The enzyme effectively cleaved fluorogenic substrate Gly-Pro-AMC with Km and Vmax of 4.578 µM and 90.84 nmoles/min, respectively. Purified DPP-IV activity was inhibited by Diprotin A with an IC

Indexed as

AnimalsDipeptidesDipeptidyl Peptidase 4Dipeptidyl-Peptidase IV InhibitorsEnzyme AssaysKineticsMolecular WeightOligopeptidesSubstrate SpecificitySwineDipeptidesDipeptidyl Peptidase 4Dipeptidyl-Peptidase IV Inhibitorsdiprotin AglycylprolineOligopeptidesAzadirachta indicaDiprotin ADPP-IVGLP-1Momordica carantiaTerminalia chebula

Identifiers

PMID32577945
OpenAlexW3035849699

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.