Evidence map›Paper›PMID 32577653›Full record

ArticlebioRxiv : the preprint server for biology2020

SARS-CoV-2 nucleocapsid protein undergoes liquid-liquid phase separation stimulated by RNA and partitions into phases of human ribonucleoproteins.

Theodora Myrto Perdikari, Anastasia C Murthy, Veronica H Ryan, Scott Watters, Mandar T Naik, Nicolas L Fawzi

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 52 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Theodora Myrto PerdikariCenter for Biomedical Engineering, Brown University, Providence, RI, USA.
Anastasia C MurthyMolecular Biology, Cell Biology & Biochemistry Graduate Program, Brown University, Providence, RI, USA.
Veronica H RyanNeuroscience Graduate Program, Brown University, Providence, RI, USA.
Scott WattersDepartment of Molecular Pharmacology, Physiology, and Biotechnology, Brown University, Providence, RI, USA.
Mandar T NaikDepartment of Molecular Pharmacology, Physiology, and Biotechnology, Brown University, Providence, RI, USA.
Nicolas L FawziDepartment of Molecular Pharmacology, Physiology, and Biotechnology, Brown University, Providence, RI, USA.
Brown University · USAllen Institute for Brain Science · US

Funding

Functional and Pathological Interactions of TDP-43R01NS116176 · NINDS · BROWN UNIVERSITY · PI FAWZI, NICOLAS LUX, MITTAL, JEETAIN · 2020 to 2024
$3.6M
Turning off the molecular switch for pathological self-assembly of FUSR01GM118530 · NIGMS · BROWN UNIVERSITY · PI FAWZI, NICOLAS LUX · 2016 to 2020
$1.6M
Effect of neurodegenerative disease mutations and post-translational modifications on hnRNPA2 structure, function, and interactions in phase-separated ribonucleoprotein granulesF31NS110301 · NINDS · BROWN UNIVERSITY · PI RYAN, VERONICA HANLEY · 2018 to 2019
$87k
NIGMS NIH HHS R01 GM118530NINDS NIH HHS F31 NS110301NINDS NIH HHS R01 NS116176
6 · The paper itself

Abstract

Tightly packed complexes of nucleocapsid protein and genomic RNA form the core of viruses and may assemble within viral factories, dynamic compartments formed within the host cells. Here, we examine the possibility that the multivalent RNA-binding nucleocapsid protein (N) from the severe acute respiratory syndrome coronavirus (SARS-CoV-2) compacts RNA via protein-RNA liquid-liquid phase separation (LLPS) and that N interactions with host RNA-binding proteins are mediated by phase separation. To this end, we created a construct expressing recombinant N fused to a N-terminal maltose binding protein tag which helps keep the oligomeric N soluble for purification. Using

Identifiers

PMID32577653
PMCPMC7302208
OpenAlexW3034808847

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.