ArticleOpen forum infectious diseases2020
Early-Morning vs Spot Posterior Oropharyngeal Saliva for Diagnosis of SARS-CoV-2 Infection: Implication of Timing of Specimen Collection for Community-Wide Screening.
Article in Open forum infectious diseases, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 2 of them syntheses that pooled it.
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Who cites it
27 citing papers in PubMed, 2 syntheses or guidelines pooled it, 40 citations in OpenAlex.
- Saliva sample for the massive screening of SARS-CoV-2 infection: a systematic review.Oral surgery, oral medicine, oral pathology and oral radiology · 2021Pooled it
- Do hydrogen peroxide mouthwashes have a virucidal effect? A systematic review.The Journal of hospital infection · 2020Pooled it
- Impact of Swabbing Location, Self-Swabbing, and Food Intake on SARS-CoV-2 RNA Detection.Microorganisms · 2024Article
- The potential of saliva as an accessible and sensitive sample type for the detection of respiratory pathogens and host immunity.The Lancet. Microbe · 2023Review
- Diagnostic Performance and Tolerability of Saliva and Nasopharyngeal Swab Specimens in the Detection of SARS-CoV-2 by RT-PCR.Microbiology spectrum · 2023Article
- Association between soluble angiotensin-converting enzyme 2 in saliva and SARS-CoV-2 infection: a cross-sectional study.Scientific reports · 2023Article
- Comparing SARS-CoV-2 Viral Load in Human Saliva to Oropharyngeal Swabs, Nasopharyngeal Swabs, and Sputum: A Systematic Review and Meta-Analysis.The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2023Review
- Morning SARS-CoV-2 Testing Yields Better Detection of Infection Due to Higher Viral Loads in Saliva and Nasal Swabs upon Waking.Microbiology spectrum · 2022Article
- Evolution and Control of COVID-19 Epidemic in Hong Kong.Viruses · 2022Review
- Saliva as a diagnostic specimen for SARS-CoV-2 detection: A scoping review.Oral diseases · 2022Article
- Article
- Does the timing of saliva collection affect the diagnosis of SARS-CoV-2 infection?Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy · 2022Article
- Salivary diagnostics of the novel coronavirus SARS-CoV-2 (COVID-19).Oral diseases · 2022Review
- Amplified parallel antigen rapid test for point-of-care salivary detection of SARS-CoV-2 with improved sensitivity.Mikrochimica acta · 2021Article
- Lessons learned 1 year after SARS-CoV-2 emergence leading to COVID-19 pandemic.Emerging microbes & infections · 2021Review
- The Use of Saliva as a Biosample in the Light of COVID-19.Diagnostics (Basel, Switzerland) · 2021Review
- Detection of SARS-CoV-2 Infection in Gargle, Spit, and Sputum Specimens.Microbiology spectrum · 2021Article
- Avoiding COVID-19 hospital outbreaks: RT-PCR swab and clinical assessment.International journal of clinical practice · 2021Article
- Saliva Exhibits High Sensitivity and Specificity for the Detection of SARS-COV-2.Diseases (Basel, Switzerland) · 2021Review
- Article
Corrections and comments
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Authors and funding
14 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPosterior oropharyngeal saliva is increasingly recognized as a valid respiratory specimen for SARS-CoV-2 diagnosis. It is easy to collect and suitable for community-wide screening. The optimal timing of collection is currently unknown, and we speculate that an early-morning specimen before oral hygiene and breakfast would increase the diagnostic yield.
methodsPosterior oropharyngeal saliva was collected at 5 different time points within the same day from 18 patients with previously confirmed SARS-CoV-2 infection by molecular testing. Cycle threshold (Ct) values were compared.
resultsThere was an overall trend of lower Ct values from specimens collected in the early morning, with a gradual decrease of viral load towards nighttime, but reaching statistical significance only when compared with the specimens collected at bedtime. Eight out of 13 subjects had a higher viral load in the early morning than the rest of the 4 time points (before lunch, before teatime at 3 pm, before dinner, before bedtime).
conclusionsThe result suggests a diurnal variation of viral shedding from the upper respiratory tract with a trend showing higher viral load in the early morning. For community screening purposes, posterior oropharyngeal saliva could be taken throughout the day, but preferably in the early morning to maximize the yield.
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