ArticleProceedings of the National Academy of Sciences of the United States of America2020
Real-time monitoring of the in vivo redox state transition using the ratiometric redox state sensor protein FROG/B.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 28 citations in OpenAlex.
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- Cold atmospheric plasma treatment enhances recombinant model protein production in yeast Pichia pastoris.Scientific reports · 2023Article
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- Article
- Genetically Encoded Fluorescent Redox Indicators for Unveiling Redox Signaling and Oxidative Toxicity.Chemical research in toxicology · 2021Review
- Live monitoring of plant redox and energy physiology with genetically encoded biosensors.Plant physiology · 2021Review
- Quantification of NAD(P)H in cyanobacterial cells by a phenol extraction method.Photosynthesis research · 2021Article
- Imaging Biomarkers for Monitoring the Inflammatory Redox Landscape in the Brain.Antioxidants (Basel, Switzerland) · 2021Review
- FROG/B reporter gives bright insights into redox biology.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The intracellular redox state is one of the key factors regulating various physiological phenomena in the cell. Monitoring this state is therefore important for understanding physiological homeostasis in cells. Various fluorescent sensor proteins have already been developed to monitor intracellular redox state. We also developed fluorescent redox sensor proteins named Oba-Q and Re-Q, the emissions of which are quenched under oxidized and reduced conditions, respectively. Although these sensors were useful to visualize the redox changes in the cell over time, they have the weakness that their emission signals are directly influenced by their in situ expression levels. To overcome this problem, we developed a redox sensor protein with a single excitation peak and dual variable emission peaks. This sensor protein shows green emission under oxidized conditions and blue emission under reduced conditions. We therefore named this sensor FROG/B, fluorescent protein with redox-dependent change in green/blue. By using this sensor, we successfully measured the changes in intracellular redox potentials in cyanobacterial cells quantitatively caused by light/dark transition just by calculating the ratio of emission between green and blue signals.
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