Evidence map›Paper›PMID 32576581›Full record

ArticleCancer genomics & proteomics

NONO Is a Negative Regulator of

Shanshan Liang, Hidehisa Takahashi, Tetsuro Hirose, Yasuhiro Kuramitsu, Shigetsugu Hatakeyama, Hironori Yoshiyama, Ruoyu Wang, Jun-Ichi Hamada, Hisashi Iizasa

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cancer genomics & proteomics. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.2field-weighted citation impact, top 53% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 7 citations in OpenAlex.

  1. Review
  2. Article
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  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 5 institutions in 2 countries.

Shanshan LiangThe Key Laboratory of Biomarker High-throughput Screening and Target Translation of Breast and Gastrointestinal Tumor, Oncology Department, Affiliated Zhongshan Hospital of Dalian University, Dalian, P.R. China.
Hidehisa TakahashiDepartment of Biochemistry, Hokkaido University Graduate School of Medicine, Sapporo, Japan.
Tetsuro HiroseDivision of RNA Bio-function, Institute for Genetic Medicine, Hokkaido University, Sapporo, Japan.
Yasuhiro KuramitsuHealth Science University of Hokkaido School of Nursing & Social Services, Hokkaido, Japan.
Shigetsugu HatakeyamaDepartment of Biochemistry, Hokkaido University Graduate School of Medicine, Sapporo, Japan.
Hironori YoshiyamaDepartment of Microbiology, Faculty of Medicine, Shimane University, Shimane, Japan.
Ruoyu WangThe Key Laboratory of Biomarker High-throughput Screening and Target Translation of Breast and Gastrointestinal Tumor, Oncology Department, Affiliated Zhongshan Hospital of Dalian University, Dalian, P.R. China.
Jun-Ichi HamadaDivision of Stem Cell Biology, Institute for Genetic Medicine, Hokkaido University, Hokkaido, Japan jun1hamada@hoku-iryo-u.ac.jp iizasah@med.shimane-u.ac.jp.
Hisashi IizasaDepartment of Microbiology, Faculty of Medicine, Shimane University, Shimane, Japan jun1hamada@hoku-iryo-u.ac.jp iizasah@med.shimane-u.ac.jp.
Affiliated Zhongshan Hospital of Dalian University · CNHealth Sciences University of Hokkaido · JPHokkaido University · JPShimane University · JPYokohama City University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

BACKGROUND/

aimSex determining region Y (SRY)-box 2 (SOX2) is a transcription factor essential for the maintenance of proliferation and self-renewal of cancer stem cells and is associated with breast cancer initiation. Regulation of cancer stem cell plasticity by SOX2 requires both positive and negative SOX2 transcription factors, but the negative regulator is still largely unknown. MATERIALS AND

methodsSOX2 promoter-binding proteins were identified by liquid chromatography-mass spectrometry/mass spectrometry, luciferase assay, and chromatin immunoprecipitation. The effects of one such transcription factor on SOX2 expression was investigated by knockdown and overexpression experiments.

resultsNon-POU domain-containing octamer-binding protein (NONO) (also known as 54-kDa nuclear RNA-binding protein, P54NRB) was identified as a SOX2 promoter-binding protein and a negative regulator of SOX2 expression. Its activity was controlled by its coiled-coil domain and the C-terminal domain.

conclusionThese results suggest that NONO acts as a key regulator of SOX2 transcription through the repression of SOX2 promoter activity in breast cancer cells.

Indexed as

Gene Expression Regulation, NeoplasticBiomarkers, TumorBreast NeoplasmsCell Line, TumorDNA-Binding ProteinsFemaleHumansNeoplastic Stem CellsPromoter Regions, GeneticRNA-Binding ProteinsSOXB1 Transcription FactorsBiomarkers, TumorDNA-Binding ProteinsNONO protein, humanRNA-Binding ProteinsSOX2 protein, humanSOXB1 Transcription Factorsbreast cancercancer stem cellnegative regulatorSOX2transcription factor

Identifiers

PMID32576581
PMCPMC7367599
OpenAlexW3037946389

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.