ArticleBMC biotechnology2020
Efficient CRISPR/Cas9 genome editing in a salmonid fish cell line using a lentivirus delivery system.
Article in BMC biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 65 citations in OpenAlex.
- Transient Trim25 Knockdown Enhances Transfection Efficiency in Two Teleost Fish Cell Lines.Marine biotechnology (New York, N.Y.) · 2026Article
- Fish cell lines in aquaculture and biomedical research: A global perspective on development, repositories, and multidisciplinary applications.Molecular biology reports · 2026Review
- Development and characterisation of a novel cell line derived from coho salmon (Oncorhynchus kisutch).In vitro cellular & developmental biology. Animal · 2026Article
- Systematic Evaluation of Electroporation Conditions for Plasmid in Pacific Oyster Crassostrea gigas Zygotes.Marine biotechnology (New York, N.Y.) · 2026Article
- Efficient Gene Editing in Fish Primary Germline Stem Cells.International journal of molecular sciences · 2026Article
- Advances in Fish Gene Editing.Animals : an open access journal from MDPI · 2026Review
- Comparative Analysis of CRISPR/Cas9 Delivery Methods in Marine Teleost Cell Lines.International journal of molecular sciences · 2025Article
- Innovative approaches to combat antibiotic resistance: integrating CRISPR/Cas9 and nanoparticles against biofilm-driven infections.BMC medicine · 2025Review
- The snakehead retrovirus promoter functions independently of the 3'ORF protein and its products are maternally inherited in transgenic zebrafish.PLoS pathogens · 2025Article
- Genome editing of WSSV CRISPR/Cas9 and immune activation extends the survival of infected Penaeus vannamei.Scientific reports · 2024Article
- CRISPR/Cas9 Technology for Enhancing Desirable Traits of Fish Species in Aquaculture.International journal of molecular sciences · 2024Review
- Generation of transgenic fish cell line with α-lactalbumin nanocarriers co-delivering Tol2 transposase mRNA and plasmids.iScience · 2024Article
- Cell-cultivated aquatic food products: emerging production systems for seafood.Journal of biological engineering · 2024Review
- Role of CRISPR-Cas systems and anti-CRISPR proteins in bacterial antibiotic resistance.Heliyon · 2024Review
- Review
- An Efficient Vector-Based CRISPR/Cas9 System in Zebrafish Cell Line.Marine biotechnology (New York, N.Y.) · 2024Article
- Review
- Fish cell line: depositories, web resources and future applications.Cytotechnology · 2024Review
- A signal recognition particle receptor gene from the sea cucumber, Apostichopus japonicas.Scientific reports · 2023Article
- Removal of evolutionarily conserved functional MYC domains in a tilapia cell line using a vector-based CRISPR/Cas9 system.Scientific reports · 2023Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundGenome editing is transforming bioscience research, but its application to non-model organisms, such as farmed animal species, requires optimisation. Salmonids are the most important aquaculture species by value, and improving genetic resistance to infectious disease is a major goal. However, use of genome editing to evaluate putative disease resistance genes in cell lines, and the use of genome-wide CRISPR screens is currently limited by a lack of available tools and techniques.
resultsIn the current study, we developed an optimised protocol using lentivirus transduction for efficient integration of constructs into the genome of a Chinook salmon (Oncorhynchus tshwaytcha) cell line (CHSE-214). As proof-of-principle, two target genes were edited with high efficiency in an EGFP-Cas9 stable CHSE cell line; specifically, the exogenous, integrated EGFP and the endogenous RIG-I locus. Finally, the effective use of antibiotic selection to enrich the successfully edited targeted population was demonstrated.
conclusionsThe optimised lentiviral-mediated CRISPR method reported here increases possibilities for efficient genome editing in salmonid cells, in particular for future applications of genome-wide CRISPR screens for disease resistance.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.