ArticleBMC microbiology2020
Development of a droplet digital PCR method for detection of Streptococcus agalactiae.
Article in BMC microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 20 citations in OpenAlex.
- Screening forBio-protocol · 2025Article
- Establishment of one-step duplex TaqMan real-time PCR for detection of feline coronavirus and panleukopenia virus.Applied microbiology and biotechnology · 2025Article
- Development of a droplet digital PCR method for the detection ofPractical laboratory medicine · 2025Article
- To evaluate the performance of simultaneous amplification and testing assay for group B Streptococcus detection: comparison with real-time PCR and ddPCR assays.Annals of clinical microbiology and antimicrobials · 2024Article
- Development and performance evaluation of a qPCR-based assay for the fully automated detection of group BMicrobiology spectrum · 2024Article
- Occurrence of 40 sanitary indicators in French digestates derived from different anaerobic digestion processes and raw organic wastes from agricultural and urban origin.Frontiers in microbiology · 2024Article
- Ultra-Fast Impedimetric Immunoassay for Detection ofMicromachines · 2023Article
- Comparison of Droplet Digital PCR and Metagenomic Next-Generation Sequencing Methods for the Detection of Human Herpesvirus 6B Infection Using Cell-Free DNA from Patients Receiving CAR-T and Hematopoietic Stem Cell Transplantation.Infection and drug resistance · 2022Article
- Current Applications of Absolute Bacterial Quantification in Microbiome Studies and Decision-Making Regarding Different Biological Questions.Microorganisms · 2021Review
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
Abstract
backgroundStreptococcus agalactiae (GBS) is the causative pathogen of puerperal sepsis in pregnant women and pneumonia, sepsis and meningitis in infants. Infection of GBS is responsible for the increased morbidity in pregnant women and the elderly, and bring challenges to clinical diagnosis and treatment. However, culture-based approaches to detect S.agalactiae is time-consuming with limited sensitivity. Besides, real-time quantitative PCR demands expensive instruments with tedious steps. Thus, we aim to establish a new detection method for more accurate and rapid detection of S.agalactiae.
resultsThe ddPCR primer targeted the CpsE gene showed better amplified efficiency in the reaction. The limit of detection for GBS DNA with ddPCR was able to reach 5 pg/μL. Moreover, no positive amplified signals could be detected in the reactions which served 11 non-GBS strains DNA as templates. Furthermore, the coefficient of variation of this method was 4.5%, indicating excellent repeatability of ddPCR assay.
conclusionsIn our study, ddPCR was performed as a rapid detection of S.agalactiae with high sensitivity and specificity. This technique can promote the accuracy of the diagnosis of GBS infection and provide a scientific basis for clinical treatment.
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