ArticleAnimals : an open access journal from MDPI2020
Changes in Bull Semen Metabolome in Relation to Cryopreservation and Fertility.
Article in Animals : an open access journal from MDPI, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 40 citations in OpenAlex.
- Influence of sire fertility on the metabolism of in vitro produced embryos.Reproduction & fertility · 2026Article
- Article
- Review
- L-Glutamine Supplementation Improves the In Vitro Qualitative Parameters of Cryopreserved Qinchuan Bull Sperm.Animals : an open access journal from MDPI · 2025Article
- Untargeted Metabolomic Identifies Potential Seasonal Biomarkers of Semen Quality in Duroc Boars.Biology · 2025Article
- Effect of Seminal Plasma on the Freezability of Boar Sperm.Animals : an open access journal from MDPI · 2024Article
- Proteomics and Metabolic Characteristics of Boar Seminal Plasma Extracellular Vesicles Reveal Biomarker Candidates Related to Sperm Motility.Journal of proteome research · 2024Article
- Risk of Sperm Disorders and Impaired Fertility in Frozen-Thawed Bull Semen: A Genome-Wide Association Study.Animals : an open access journal from MDPI · 2024Article
- Effect of cryopreservation and semen extender on extracellular vesicles isolated from bull semen.Frontiers in veterinary science · 2024Article
- Abnormalities in mitochondrial energy metabolism induced by cryopreservation negatively affect goat sperm motility.Frontiers in veterinary science · 2024Article
- Integration of omics studies indicates that species-dependent molecular mechanisms govern male fertility.Journal of animal science and biotechnology · 2023Article
- Factors affecting theVeterinarni medicina · 2023Review
- Buffalo milk and rumen fluid metabolome are significantly affected by green feed.Scientific reports · 2023Article
- Alginate oligosaccharide extends the service lifespan by improving the sperm metabolome and gut microbiota in an aging Duroc boars model.Frontiers in cellular and infection microbiology · 2023Article
- The future of assessing bull fertility: Can the 'omics fields identify usable biomarkers?†.Biology of reproduction · 2022Review
- Metabolomic analysis reveals spermatozoa and seminal plasma differences between Duroc and Liang guang Small-spotted pig.Frontiers in veterinary science · 2022Article
- Evaluation of lipidomic change in goat sperm after cryopreservation.Frontiers in veterinary science · 2022Article
- Identification of biomarkers for bull fertility using functional genomics.Animal reproduction · 2022Article
- Effect of insulin-like growth factor-1 complex of Simmental bull seminal plasma on post-thawed Kacang buck semen fertility.Veterinary world · 2021Article
- Sperm Metabolomics through Nuclear Magnetic Resonance Spectroscopy.Animals : an open access journal from MDPI · 2021Review
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
Semen cryopreservation determines several sperm damages, including the loss of fertility-associated proteins. The purpose of the study was to compare the metabolite contents in bovine sperm and seminal plasma before and after cryopreservation, and between high- and low-fertility bulls in vitro. Forty-eight ejaculates, collected from eight bulls (six per bull), were analyzed by liquid chromatography-mass spectrometry. Cryopreservation resulted in an over-expression of lysophosphatidylcholine (0:0/18:2(9Z,12Z)) in seminal plasma. In addition, higher levels of glycine betaine and pyro-l-glutaminyl-l-glutamine were observed in cryopreserved compared to fresh spermatozoa. The fresh seminal plasma of high-fertility bulls showed an over-expression of l-acetylcarnitine, glycerol tripropanoate, 2,3-diacetoxypropyl stearate and glycerophosphocholine, and an under-expression of lysophosphatidylcholine and butyrylcarnitine, compared to low-fertility bulls. Higher levels of glycerophosphocholine and lysophosphatidylcholine (16:0/0:0) were recorded in fresh spermatozoa from high-fertility bulls. In high-fertility bulls, a greater content of glycerophosphocholine and lower levels of butyrylcarnitine, glycine betaine and l-carnitine were found in cryopreserved seminal plasma, and lower levels of glycine betaine were detected in cryopreserved spermatozoa. In conclusion, cryopreservation affects bovine semen metabolome at both plasmatic and cellular compartments, and metabolic profile differs between high- and low-fertility bulls.
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Registered trials
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