Evidence map›Paper›PMID 32575635›Full record

ArticleInternational journal of molecular sciences2020

Proteome Analysis Reveals Syndecan 1 Regulates Porcine Sapelovirus Replication.

Tingting Zhao, Li Cui, Xiangqian Yu, Zhonghai Zhang, Qi Chen, Xiuguo Hua

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 56% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Tingting ZhaoShanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
Li CuiShanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.ORCID 0000-0001-5202-9671
Xiangqian YuShanghai Pudong New Area Center for Animal Disease Control and Prevention, Shanghai 200136, China.
Zhonghai ZhangShanghai Pudong New Area Center for Animal Disease Control and Prevention, Shanghai 200136, China.
Qi ChenShanghai Animal Disease Control Center, Shanghai 201103, China.
Xiuguo HuaShanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
Shanghai Jiao Tong University · CNShanghai Municipal Center For Disease Control Prevention · CNChina Animal Disease Control Center · CN

Funding

National Basic Research Program of China (973 Program) No.2017YFC1200203National Natural Science Foundation of China 31572525
6 · The paper itself

Abstract

Porcine sapelovirus A (PSV) is a single stranded, positive-sense, non-enveloped RNA virus that causes enteritis, pneumonia, polioencephalomyelitis, and reproductive disorders in pigs. Research on PSV infection and interaction with host cells is unclear. In this study, we applied tandem mass tag proteomics analysis to investigate the differentially expressed proteins (DEPs) in PSV-infected pig kidney (PK)-15 cells and explored the interactions between PSV and host cells. Here we mapped 181 DEPs, including 59 up-regulated and 122 down-regulated DEPs. Among them, osteopontin (SPP1), induced protein with tetratricopeptide repeats 5 (IFIT5), ISG15 ubiquitin-like modifier (ISG15), vinculin (VCL), and syndecan-1 (SDC1) were verified significantly changed using RT-qPCR. Additionally, overexpression of SDC1 promoted PSV viral protein (VP)1 synthesis and virus titer, and silencing of SDC1 revealed the opposite results. Our findings show that SDC1 is a novel host protein and plays crucial roles in regulating PSV replication.

Indexed as

AnimalsCell LineGene Expression RegulationModels, BiologicalPicornaviridaePicornaviridae InfectionsProteomicsSwineSwine DiseasesSyndecan-1Tandem Mass SpectrometryViral LoadViral ProteinsVirus ReplicationSyndecan-1Viral Proteinsquantitative proteomicsreplicationsapelovirustandem mass tag

Identifiers

PMID32575635
PMCPMC7352226
OpenAlexW3036101114

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.