ArticleNeuron2020
Soma-Targeted Imaging of Neural Circuits by Ribosome Tethering.
Article in Neuron, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed, 83 citations in OpenAlex.
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- Light-field deep learning enables high-throughput, scattering-mitigated calcium imaging.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Combinatorial responsiveness of chemosensory neurons in mouse explants revealed by DynamicNeuroTracker.Cell reports methods · 2025Article
- Synthetic multicolor antigen-stabilizable nanobody platform for intersectional labelling and functional imaging.bioRxiv : the preprint server for biology · 2025Article
- Striatal astrocytes modulate behavioral flexibility and whole-body metabolism in mice.Nature communications · 2025Article
- Preserving spatial and quantitative information in unpaired biomedical image-to-image translation.Cell reports methods · 2025Article
- A sensitive soma-localized red fluorescent calcium indicator for in vivo imaging of neuronal populations at single-cell resolution.PLoS biology · 2025Article
- Higher-order thalamic input to cortex selectively conveys state information.Cell reports · 2025Article
- Population imaging of internal state circuits relevant to psychiatric disease: a review.Neurophotonics · 2025Review
- Identifying dysfunctional cell types and circuits in animal models for psychiatric disorders with calcium imaging.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2024Review
- Photonic neural probe enabled microendoscopes for light-sheet light-field computational fluorescence brain imaging.Neurophotonics · 2024Article
- Scanless two-photon voltage imaging.Nature communications · 2024Article
- Updated Toolbox for Assessing Neuronal Network Reconstruction after Cell Therapy.Bioengineering (Basel, Switzerland) · 2024Review
- A multicolor suite for deciphering population coding of calcium and cAMP in vivo.Nature methods · 2024Article
- Multibeam continuous axial scanning two-photon microscopy forBiomedical optics express · 2024Article
- Article
- Advancing spinal cord injury research with optical clearing, light sheet microscopy, and artificial intelligence-based image analysis.Neural regeneration research · 2023Article
- Genetic Approaches for Neural Circuits Dissection in Non-human Primates.Neuroscience bulletin · 2023Review
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17 authors at 3 institutions in 1 country.
Funding
Abstract
Neuroscience relies on techniques for imaging the structure and dynamics of neural circuits, but the cell bodies of individual neurons are often obscured by overlapping fluorescence from axons and dendrites in surrounding neuropil. Here, we describe two strategies for using the ribosome to restrict the expression of fluorescent proteins to the neuronal soma. We show first that a ribosome-tethered nanobody can be used to trap GFP in the cell body, thereby enabling direct visualization of previously undetectable GFP fluorescence. We then design a ribosome-tethered GCaMP for imaging calcium dynamics. We show that this reporter faithfully tracks somatic calcium dynamics in the mouse brain while eliminating cross-talk between neurons caused by contaminating neuropil. In worms, this reporter enables whole-brain imaging with faster kinetics and brighter fluorescence than commonly used nuclear GCaMPs. These two approaches provide a general way to enhance the specificity of imaging in neurobiology.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.