Evidence map›Paper›PMID 32572674›Full record

ArticleJournal of assisted reproduction and genetics2020

Profiling the DNA methylation patterns of imprinted genes in abnormal semen samples by next-generation bisulfite sequencing.

Wanhong He, Υuhua Sun, Sufen Zhang, Xing Feng, Minjie Xu, Jianfeng Dai, Xiaohua Ni, Xin Wang, Qihan Wu

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of assisted reproduction and genetics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
2.2field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 21 citations in OpenAlex.

  1. Methods for Investigating the Sperm Epigenome.Advances in experimental medicine and biology · 2026
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  17. Unraveling the Molecular Impact of Sperm DNA Damage on Human Reproduction.Advances in experimental medicine and biology · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Wanhong HeNHC Key Lab of Reproduction Regulation (Shanghai Institute of Planned Parenthood Research), Fudan University, Shanghai, China.
Υuhua SunDepartment of Laboratory Medicine, Shanghai Institute of Planned Parenthood Research Hospital, SIPPR, Shanghai, China.
Sufen ZhangNHC Key Lab of Reproduction Regulation (Shanghai Institute of Planned Parenthood Research), Fudan University, Shanghai, China.
Xing FengDepartment of Laboratory Medicine, Shanghai Institute of Planned Parenthood Research Hospital, SIPPR, Shanghai, China.
Minjie XuNHC Key Lab of Reproduction Regulation (Shanghai Institute of Planned Parenthood Research), Fudan University, Shanghai, China.
Jianfeng DaiInstitutes of Biology and Medical Sciences, Jiangsu Key Laboratory of Infection and Immunity, Soochow University, Suzhou, China.
Xiaohua NiNHC Key Lab of Reproduction Regulation (Shanghai Institute of Planned Parenthood Research), Fudan University, Shanghai, China.
Xin WangDepartment of Laboratory Medicine, Shanghai Institute of Planned Parenthood Research Hospital, SIPPR, Shanghai, China. wangxin51305@126.com.
Qihan WuNHC Key Lab of Reproduction Regulation (Shanghai Institute of Planned Parenthood Research), Fudan University, Shanghai, China. henrywuqh@hotmail.com.ORCID http://orcid.org/0000-0002-6529-4877
Fudan University · CNShanghai Institute of Planned Parenthood Research · CNSoochow University · CN

Funding

Major Research Plan 81872103Major Research Plan 81971917Science and Technology Climbing Fund of SIPPR PD2017-2Science and Technology Climbing Fund of SIPPR PD2017-4
6 · The paper itself

Abstract

purposeChanges in DNA methylation modifications have been associated with male infertility. With the development of assisted reproductive technologies (ARTs), abnormal DNA methylation in sperm, especially in imprinted genes, may impact the health of offspring and requires an in-depth study.

methodsIn this study, we collected abnormal human semen samples, including asthenospermic, oligospermic, oligoasthenospermic and deformed sperm, and investigated the methylation of imprinted genes by reduced representation bisulfite sequencing (RRBS) and bisulfite amplicon sequencing on the Illumina platform.

resultsThe differentially methylated regions (DMRs) of imprinted genes, including H19, GNAS, MEG8 and SNRPN, were different in the abnormal semen groups. MEG8 DMR methylation in the asthenospermic group was significantly increased. Furthermore, higher methylation levels of MEG8, GNAS and SNRPN DMR in the oligospermic and oligoasthenospermic groups and a decrease in the H19 DMR methylation level in the oligospermic group were observed. However, the methylation levels of these regions varied greatly among the different semen samples and among individual sperm within the same semen sample. The SNP rs2525883 genotype in the H19 DMR affected DNA methylation. Moreover, DNA methylation levels differed in the abnormal semen groups in the non-imprinted genomic regions, including repetitive sequence DNA transposons and long/short interspersed nuclear elements (LINEs and SINEs).

conclusionOur study established that imprinted gene DMRs, such as H19, GNAS, SNRPN and MEG8, were differentially methylated in the abnormal semen groups with obvious inter- and intra-sample heterogeneities. These results suggest that special attention needs to be paid to possible epigenetic risks during reproduction.

Indexed as

AdultAsthenozoospermiaChromograninsDNA MethylationEpigenomicsGene Expression Regulation, DevelopmentalGenomic ImprintingGTP-Binding Protein alpha Subunits, GsHigh-Throughput Nucleotide SequencingHumansInfertility, MaleMaleMiddle AgedRNA, Long NoncodingSemensnRNP Core ProteinsChromograninsGNAS protein, humanGTP-Binding Protein alpha Subunits, GsH19 long non-coding RNARNA, Long NoncodingsnRNP Core ProteinsDNA methylationImprinted genesSpermatozoa

Identifiers

PMID32572674
PMCPMC7492341
OpenAlexW3037872887

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.