ArticleScientific reports2020
An integrative DNA barcoding framework of ladybird beetles (Coleoptera: Coccinellidae).
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 39 citations in OpenAlex.
- Mitochondrial Genome Comparison and Phylogenetic Analysis of the Family Carabidae (Coleoptera: Adephaga).Biology · 2026Article
- High utility of DNA barcoding for species identification and cryptic diversity in Korean aphids (Hemiptera: Aphididae).Scientific reports · 2026Article
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- Comparative and Phylogenetic Analyses of Mitochondrial Genomes in Carabidae (Coleoptera: Adephaga).Ecology and evolution · 2025Article
- Comparative Mitogenomes and Phylogenetic Analyses of Coccinellidae (Coleoptera: Coccinelloidea).Ecology and evolution · 2025Article
- Overview and limitations of database in global traditional medicines: A narrative review.Acta pharmacologica Sinica · 2025Review
- The impact of landscape and prey on psyllophagous ladybird communities in a tropical environment.PloS one · 2025Article
- Delimiting species, revealing cryptic diversity, and population divergence in Qinghai-Tibet Plateau weevils through DNA barcoding.Ecology and evolution · 2024Article
- A Review of the Beetle GenusZoological studies · 2024Article
- Molecular characterization of mitochondrial COI gene sequences in Micraspis allardi from Pakistan.PloS one · 2023Article
- Identifying the Genetic Distance Threshold for Entiminae (Coleoptera: Curculionidae) Species Delimitation via COI Barcodes.Insects · 2022Article
- Genome Skimming Contributes to Clarifying Species Limits inFrontiers in plant science · 2022Article
- Cryptic Diversity inPathogens (Basel, Switzerland) · 2021Article
- Article
- DNA barcodes for Aotearoa New Zealand Pyraloidea (Lepidoptera).Biodiversity data journal · 2020Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Even though ladybirds are well known as economically important biological control agents, an integrative framework of DNA barcoding research was not available for the family so far. We designed and present a set of efficient mini-barcoding primers to recover full DNA barcoding sequences for Coccinellidae, even for specimens collected 40 years ago. Based on these mini-barcoding primers, we obtained 104 full DNA barcode sequences for 104 species of Coccinellidae, in which 101 barcodes were newly reported for the first time. We also downloaded 870 COI barcode sequences (658 bp) from GenBank and BOLD database, belonging to 108 species within 46 genera, to assess the optimum genetic distance threshold and compare four methods of species delimitation (GMYC, bPTP, BIN and ABGD) to determine the most accurate approach for the family. The results suggested the existence of a 'barcode gap' and that 3% is likely an appropriate genetic distance threshold to delimit species of Coccinellidae using DNA barcodes. Species delimitation analyses confirm ABGD as an accurate and efficient approach, more suitable than the other three methods. Our research provides an integrative framework for DNA barcoding and descriptions of new taxa in Coccinellidae. Our results enrich DNA barcoding public reference libraries, including data for Chinese coccinellids. This will facilitate taxonomic identification and biodiversity monitoring of ladybirds using metabarcoding.
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