Evidence map›Paper›PMID 32571933›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2020

Cell stemness is maintained upon concurrent expression of RB and the mitochondrial ribosomal protein S18-2.

Muhammad Mushtaq, Larysa Kovalevska, Suhas Darekar, Alexandra Abramsson, Henrik Zetterberg, Vladimir Kashuba, George Klein, Marie Arsenian-Henriksson, Elena Kashuba

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
1.4field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 31 citations in OpenAlex.

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  14. G1-phase progression in pluripotent stem cells.Cellular and molecular life sciences : CMLS · 2021
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 3 countries.

Muhammad MushtaqDepartment of Microbiology, Tumor and Cell Biology, Biomedicum, Karolinska Institutet, SE-171 65, Stockholm, Sweden.ORCID 0000-0002-0896-3720
Larysa KovalevskaLaboratory of Molecular Mechanisms of Cell Transformation, RE Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology of National Academy of Sciences of Ukraine, UA-03022 Kyiv, Ukraine.
Suhas DarekarDepartment of Microbiology, Tumor and Cell Biology, Biomedicum, Karolinska Institutet, SE-171 65, Stockholm, Sweden.ORCID 0000-0003-4895-3313
Alexandra AbramssonInstitute of Neuroscience and Physiology, Sahlgrenska Academy at the University of Gothenburg, SE-405 30 Gothenburg, Sweden.ORCID 0000-0002-4715-9225
Henrik ZetterbergInstitute of Neuroscience and Physiology, Sahlgrenska Academy at the University of Gothenburg, SE-405 30 Gothenburg, Sweden.
Vladimir KashubaDepartment of Microbiology, Tumor and Cell Biology, Biomedicum, Karolinska Institutet, SE-171 65, Stockholm, Sweden.
George KleinDepartment of Microbiology, Tumor and Cell Biology, Biomedicum, Karolinska Institutet, SE-171 65, Stockholm, Sweden.
Marie Arsenian-HenrikssonDepartment of Microbiology, Tumor and Cell Biology, Biomedicum, Karolinska Institutet, SE-171 65, Stockholm, Sweden.ORCID 0000-0001-6376-7792
Elena KashubaDepartment of Microbiology, Tumor and Cell Biology, Biomedicum, Karolinska Institutet, SE-171 65, Stockholm, Sweden; elena.kashuba@ki.se.ORCID 0000-0001-7001-4035
Karolinska Institutet · SENational Academy of Sciences of Ukraine · UAUniversity of Gothenburg · SEBalochistan University of Information Technology, Engineering and Management Sciences · PK

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Stemness encompasses the capability of a cell for self-renewal and differentiation. The stem cell maintains a balance between proliferation, quiescence, and regeneration via interactions with the microenvironment. Previously, we showed that ectopic expression of the mitochondrial ribosomal protein S18-2 (MRPS18-2) led to immortalization of primary fibroblasts, accompanied by induction of an embryonic stem cell (ESC) phenotype. Moreover, we demonstrated interaction between S18-2 and the retinoblastoma-associated protein (RB) and hypothesized that the simultaneous expression of RB and S18-2 is essential for maintaining cell stemness. Here, we experimentally investigated the role of S18-2 in cell stemness and differentiation. Concurrent expression of RB and S18-2 resulted in immortalization of

Indexed as

AnimalsCell DifferentiationCell ProliferationCell Self RenewalFibroblastsGene Expression Regulation, DevelopmentalHistonesHuman Embryonic Stem CellsHumansKruppel-Like Factor 4Kruppel-Like Transcription FactorsMiceMitochondriaMouse Embryonic Stem CellsPolycomb Repressive Complex 1Retinoblastoma Binding ProteinsHistonesKLF4 protein, humanKlf4 protein, mouseKruppel-Like Factor 4Kruppel-Like Transcription FactorsPolycomb Repressive Complex 1Rb1 protein, mouseRetinoblastoma Binding ProteinsRibosomal Proteinsribosomal protein S18Rnf2 protein, mouseUbiquitin-Protein Ligasescell immortalizationembryogenesisstemness and differentiationtumorigenesis

Identifiers

PMID32571933
PMCPMC7355020
OpenAlexW3036504692

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.