ArticleParasites & vectors2020
Development of a multiplex qPCR-based approach for the diagnosis of Dirofilaria immitis, D. repens and Acanthocheilonema reconditum.
Article in Parasites & vectors, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
50 citing papers in PubMed, 63 citations in OpenAlex.
- Fluorescence videography for the rapid and automated in-clinic detection of microfilariae.Parasites & vectors · 2026Article
- Improved specificity of a probe-based real-time PCR for the detection of Dirofilaria immitis in canine blood.Parasites & vectors · 2026Article
- Adulticide treatment with melarsomine: outcome in 283 heartworm-positive dogs in Germany.Parasites & vectors · 2026Article
- Molecular characterization of filarial infections and their associated risk factors in rural community of Southeastern Gabon.Parasite epidemiology and control · 2026Article
- Rapid Point-of-Care Detection ofAnimals : an open access journal from MDPI · 2026Article
- Comparative performance of the novel, point-of-care Pluslife Mini Dock Dirofilaria immitis/Dirofilaria repens detection test with the modified Knott's test in dogs.Parasites & vectors · 2026Article
- Transient Intraperitoneal Residence ofPathogens (Basel, Switzerland) · 2026Article
- Molecular Characterization ofJournal of parasitology research · 2026Article
- The evolution of diagnostic microbiology: integrating culture-based methods and genomic advances.PeerJ · 2026Review
- Global prevalence, diagnostics and epidemiological trends of canine filariosis and associated zoonotic infections (2021-2025): a review.Veterinary research communications · 2025Review
- Pathogens spread by high-flying wind-borne mosquitoes.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Nasolabial dirofilariasis in humans: a case report.Oral and maxillofacial surgery · 2025Article
- Circulation ofLife (Basel, Switzerland) · 2025Article
- Do not let your guard down! Prevalence of Dirofilaria immitis and Dirofilaria repens in dogs entering shelters in northern Italy.Parasites & vectors · 2025Article
- High seroprevalence of selected vector-borne pathogens in dogs from Saipan, Northern Mariana Islands.Parasites & vectors · 2025Article
- Article
- Molecular screening of piroplasms and Anaplasmataceae agents in Hyalomma dromedarii ticks from camels over different seasons in Egypt.Experimental & applied acarology · 2024Article
- Molecular Discovery of Filarial Nematode DNA in an Endangered Wild Pinniped (Galapagos Sea Lion,Ecology and evolution · 2024Article
- DetectingPathogens (Basel, Switzerland) · 2024Review
- Evaluation of diagnostic techniques for early detection of heartworm in experimentally infected dogs: identification of Dirofilaria immitis-derived microRNA in the initial 28 weeks post-inoculation.Parasites & vectors · 2024Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDirofilaria immitis, D. repens and Acanthocheilonema reconditum are the main causative agents of zoonotic canine filariosis.
methodsWe developed a combined multiplex approach for filaria and Wolbachia detection using the 28S-based pan-filarial and 16S-based pan-Wolbachia qPCRs, respectively, involving a fast typing method of positive samples using triplex qPCR targeting A. reconditum, D. immitis and D. repens, and a duplex qPCR targeting Wolbachia of D. immitis and D. repens. The approach was complemented by a duplex qPCR for the differential diagnosis of heartworms (D. immitis and Angiostrongylus vasorum) and pan-filarial cox1 and pan-Wolbachia ftsZ PCRs to identify other filarial parasites and their Wolbachia, respectively. A total of 168 canine blood and sera samples were used to validate the approach. Spearman's correlation was used to assess the association between filarial species and the strain of Wolbachia. Positive samples for both the heartworm antigen-test after heating sera and at least one DNA-positive for D. immitis and its Wolbachia were considered true positive for heartworm infection. Indeed, the presence of D. repens DNA or that of its Wolbachia as well as A. reconditum DNA indicates true positive infections.
resultsThe detection limit for Wolbachia and filariae qPCRs ranged from 5 × 10
conclusionsThe proposed approach is a reliable tool for the exploration and diagnosis of occult and non-occult canine filariosis. The current diagnosis of heartworm disease based on antigen detection should always be confirmed by qPCR essays. Sera heat pre-treatment is not effective and strongly discouraged.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.