ArticleBMC biotechnology2020
Loop-mediated isothermal amplification (LAMP) reaction as viable PCR substitute for diagnostic applications: a comparative analysis study of LAMP, conventional PCR, nested PCR (nPCR) and real-time PCR (qPCR) based on Entamoeba histolytica DNA derived from faecal sample.
Article in BMC biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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50 citing papers in PubMed.
- Amplification of RNA for identification of Zika and HCV in whole blood.Science advances · 2026Article
- Development loop-mediated isothermal amplification assay for detection of enteropathogenic Escherichia coli (EPEC).Molecular biology reports · 2026Article
- Application of LAMP and TaqMan qPCR for the rapid diagnosis of Anaplasma Capra (an emerging tick-borne zoonotic pathogen) and comparison with Nested-PCR.Veterinary research communications · 2026Article
- A Species-SpecificBiology · 2026Article
- The Development and Evaluation of a Loop-Mediated Isothermal Amplification (LAMP) Method for the Detection ofVeterinary sciences · 2026Article
- Evolution ofJournal of parasitology research · 2026Review
- Development and optimization of an easy to interpret loop-mediated isothermal amplification (LAMP) assay for the identification of bacterial pathogens causing childhood pneumonia.Frontiers in microbiology · 2026Article
- Advances in the detection of Drug-Resistant bacteria: current trends and innovations.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026Review
- Comparative evaluation of 14Frontiers in microbiology · 2026Article
- Evaluation of Each ThreeMicroorganisms · 2025Article
- Loop-Mediated Isothermal Amplification Assay for Visual Detection ofFoods (Basel, Switzerland) · 2025Article
- Lab-on-paper for molecular testing with USB-powered isothermal amplification and fluidic control.Mikrochimica acta · 2025Article
- PathCrisp: an innovative molecular diagnostic tool for early detection of NDM-resistant infections.Scientific reports · 2025Article
- Establishment and application of a loop-mediated isothermal amplification method based onFrontiers in veterinary science · 2025Article
- Enhanced Specificity in Colorimetric LAMP Assay forJournal of fungi (Basel, Switzerland) · 2024Article
- eDNA-Based Survey of Fish Species in Water Bodies Using Loop-Mediated Isothermal Amplification (LAMP) for Application of Developing Automatic Sampler.Methods and protocols · 2024Article
- Loop-Mediated Isothermal Amplification for Diagnosis of Zoonotic Malaria.The American journal of tropical medicine and hygiene · 2024Article
- A rapid on-site loop-mediated isothermal amplification technology as an early warning system for the detection of Shiga toxin-producingMicrobiology (Reading, England) · 2024Article
- Molecular detection of toxoplasmosis in wild rats using loop-mediated isothermal amplification assay.Veterinary world · 2024Article
- Efficient DNA Coding Algorithm for Polymerase Chain Reaction Amplification Information Retrieval.International journal of molecular sciences · 2024Article
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7 authors.
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Abstract
backgroundThis study reports the analytical sensitivity and specificity of a Loop-mediated isothermal amplification (LAMP) and compares its amplification performance with conventional PCR, nested PCR (nPCR) and real-time PCR (qPCR). All the assays demonstrated in this study were developed based on Serine-rich Entamoeba histolytica protein (SREHP) gene as study model.
resultsA set of SREHP gene specific LAMP primers were designed for the specific detection of Entamoeba histolytica. This set of primers recorded 100% specificity when it was evaluated against 3 medically important Entamoeba species and 75 other pathogenic microorganisms. These primers were later modified for conventional PCR, nPCR and qPCR applications. Besides, 3 different post-LAMP analyses including agarose gel electrophoresis, nucleic acid lateral flow immunoassay and calcein-manganese dye techniques were used to compare their limit of detection (LoD). One E. histolytica trophozoite was recorded as the LoD for all the 3 post-LAMP analysis methods when tested with E. histolytica DNA extracted from spiked stool samples. In contrast, none of the PCR method outperformed LAMP as both qPCR and nPCR recorded LoD of 100 trophozoites while the LoD of conventional PCR was 1000 trophozoites.
conclusionsThe analytical sensitivity comparison among the conventional PCR, nPCR, qPCR and LAMP reveals that the LAMP outperformed the others in terms of LoD and amplification time. Hence, LAMP is a relevant alternative DNA-based amplification platform for sensitive and specific detection of pathogens.
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