Evidence map›Paper›PMID 32570941›Full record

ArticleSensors (Basel, Switzerland)2020

Vibrational Spectroscopy as a Sensitive Probe for the Chemistry of Intra-Phase Bacterial Growth.

Kamila Kochan, Elizabeth Lai, Zack Richardson, Cara Nethercott, Anton Y Peleg, Philip Heraud, Bayden R Wood

Open access · goldAbstract read
In one paragraph

Article in Sensors (Basel, Switzerland), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
3.2field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 29 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Characterization of theInternational journal of molecular sciences · 2026
    Article
  5. Review
  6. Article
  7. Article
  8. Article
  9. Interactions ofMembranes · 2023
    Article
  10. Review
  11. Article
  12. Toxicity mechanism of CuJournal of biological physics · 2020
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Kamila KochanCentre for Biospectroscopy and School of Chemistry, Clayton Campus, Monash University, Clayton, VIC 3800, Australia.ORCID 0000-0002-7248-8637
Elizabeth LaiCentre for Biospectroscopy and School of Chemistry, Clayton Campus, Monash University, Clayton, VIC 3800, Australia.
Zack RichardsonCentre for Biospectroscopy and School of Chemistry, Clayton Campus, Monash University, Clayton, VIC 3800, Australia.
Cara NethercottInfection and Immunity Program, Monash Biomedicine Discovery Institute and Department of Microbiology, Clayton Campus, Monash University, Clayton, VIC 3800, Australia.
Anton Y PelegInfection and Immunity Program, Monash Biomedicine Discovery Institute and Department of Microbiology, Clayton Campus, Monash University, Clayton, VIC 3800, Australia.ORCID 0000-0002-2296-2126
Philip HeraudCentre for Biospectroscopy and School of Chemistry, Clayton Campus, Monash University, Clayton, VIC 3800, Australia.
Bayden R WoodCentre for Biospectroscopy and School of Chemistry, Clayton Campus, Monash University, Clayton, VIC 3800, Australia.
Monash University · AUAustralian Regenerative Medicine Institute · AU

Funding

Australian Research Council DP180103484
6 · The paper itself

Abstract

Bacterial growth in batch cultures occurs in four phases (lag, exponential/log, stationary and death phase) that differ distinctly in number of different bacteria, biochemistry and physiology. Knowledge regarding the growth phase and its kinetics is essential for bacterial research, especially in taxonomic identification and monitoring drug interactions. However, the conventional methods by which to assess microbial growth are based only on cell counting or optical density, without any insight into the biochemistry of cells or processes. Both Raman and Fourier transform infrared (FTIR) spectroscopy have shown potential to determine the chemical changes occurring between different bacterial growth phases. Here, we extend the application of spectroscopy and for the first time combine both Raman and FTIR microscopy in a multimodal approach to detect changes in the chemical compositions of bacteria within the same phase (intra-phase). We found a number of spectral markers associated with nucleic acids (IR: 964, 1082, 1215 cm

Indexed as

BacteriaCarbohydratesProteinsPrincipal Component AnalysisSpectroscopy, Fourier Transform InfraredSpectrum Analysis, RamanVibrationCarbohydratesProteinsATRgrowth phaselag phaseRamanS. aureus

Identifiers

PMID32570941
PMCPMC7348983
OpenAlexW3036394019

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.