ArticleThe Journal of international medical research2020
Effect of preservation time of formalin-fixed paraffin-embedded tissues on extractable DNA and RNA quantity.
Article in The Journal of international medical research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.
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Who cites it
29 citing papers in PubMed, 1 synthesis or guideline pooled it, 52 citations in OpenAlex.
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- Generative and integrative modeling for transcriptomics with formalin fixed paraffin embedded material.Journal of translational medicine · 2025Article
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- Examining the Formalin Fixation Method for Maintaining High RNA Quality in Surgical Lung Specimens.Thoracic cancer · 2025Article
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- Genetic screening of FFPE breast cancer biopsies for theRevista panamericana de salud publica = Pan American journal of public health · 2025Article
- Across the space: applications of spatial transcriptomic technology in healthy and diseased muscle.Frontiers in cell and developmental biology · 2025Review
- DNA extraction from long-term preserved formalin-fixed paraffin-embedded samples of thymic epithelial tumours - a performance comparison of three commercial kits.Contemporary oncology (Poznan, Poland) · 2025Article
- snRNA-seq of long-preserved FFPE samples from colorectal liver metastasis lesions with diverse prognoses.Scientific data · 2024Article
- Evidence of rustrela virus-associated feline staggering disease in Sweden since the 1970s.Acta veterinaria Scandinavica · 2024Article
- Assessing the effect of sample storage time on viral detection using a rapid and cost-effective CTAB-based extraction method.Plant methods · 2024Article
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Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesThis study aimed to investigate the factors affecting the quantity of DNA and RNA extractable from human formalin-fixed paraffin-embedded (FFPE) tissues stored for different lengths of time.
methodsWe randomly selected 20 FFPE specimens harvested from hysteromyoma patients with uterine fibroids during 2010, 2015, and 2017 at the Department of Pathology, Jiading District Central Hospital Affiliated Shanghai University of Medicine and Health Sciences. DNA and RNA extractions were performed using a DNA/RNA FFPE kit. DNA and RNA concentrations and their OD
resultsSpecimens that were stored for longer showed more degradation and a reduced concentration of DNA and RNA after nucleic acid extraction. However, there was no significant difference in DNA or RNA purity. β-globin and ALDH
conclusionWe found that FFPE tissues stored for longer had a reduced quantity of extractable DNA and RNA. However, these tissues could be used for the analysis of some small target genes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.