ArticleApplied microbiology and biotechnology2020
Characterization of highly active 2-keto-3-deoxy-L-arabinonate and 2-keto-3-deoxy-D-xylonate dehydratases in terms of the biotransformation of hemicellulose sugars to chemicals.
Article in Applied microbiology and biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed, 8 citations in OpenAlex.
- Investigation of Substrate Specificity in 5-Keto-4-Deoxy-Glucarate Dehydratase and 2-Keto-3-Deoxy-L-Arabinonate Dehydratase.Chembiochem : a European journal of chemical biology · 2026Article
- Plug-and-play - enzymatic amino acid production from methanol and carbon dioxide.Nature communications · 2026Article
- Recent advances in pathway engineering for the biosynthesis of 1,4-butanediol.Applied microbiology and biotechnology · 2026Review
- Engineering Saccharomyces cerevisiae for growth on xylose using an oxidative pathway.Applied microbiology and biotechnology · 2025Article
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 2 countries.
Funding
Abstract
2-keto-3-L-arabinonate dehydratase (L-KdpD) and 2-keto-3-D-xylonate dehydratase (D-KdpD) are the third enzymes in the Weimberg pathway catalyzing the dehydration of respective 2-keto-3-deoxy sugar acids (KDP) to α-ketoglutaric semialdehyde (KGSA). The Weimberg pathway has been explored recently with respect to the synthesis of chemicals from L-arabinose and D-xylose. However, only limited work has been done toward characterizing these two enzymes. In this work, several new L-KdpDs and D-KdpDs were cloned and heterologously expressed in Escherichia coli. Following kinetic characterizations and kinetic stability studies, the L-KdpD from Cupriavidus necator (CnL-KdpD) and D-KdpD from Pseudomonas putida (PpD-KdpD) appeared to be the most promising variants from each enzyme class. Magnesium had no effect on CnL-KdpD, whereas increased activity and stability were observed for PpD-KdpD in the presence of Mg
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