Evidence map›Paper›PMID 32566991›Full record

ArticleMolecular genetics and genomics : MGG2020

Characterization and application of a putative transcription factor (SUT2) in Pichia pastoris.

Yankun Yang, Yating Zheng, Pengcheng Wang, Xiang Li, Chunjun Zhan, Robert J Linhardt, Fuming Zhang, Xiuxia Liu, Jinling Zhan, Zhonghu Bai

Open access · greenAbstract read
PubMed Publisher
In one paragraph

Article in Molecular genetics and genomics : MGG, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.4field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 7 citations in OpenAlex.

  1. Mediator Engineering ofApplied and environmental microbiology · 2022
    Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 3 countries.

Yankun YangKey Laboratory of Carbohydrate Chemistry and Biotechnology, School of Biotechnology, Jiangnan University, Wuxi, 214122, China. yangyankun@jiangnan.edu.cn.ORCID http://orcid.org/0000-0003-4815-6832
Yating ZhengKey Laboratory of Carbohydrate Chemistry and Biotechnology, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.
Pengcheng WangKey Laboratory of Carbohydrate Chemistry and Biotechnology, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.
Xiang LiKey Laboratory of Carbohydrate Chemistry and Biotechnology, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.
Chunjun ZhanKey Laboratory of Carbohydrate Chemistry and Biotechnology, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.
Robert J LinhardtCenter for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY, 12180, USA.
Fuming ZhangCenter for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY, 12180, USA.
Xiuxia LiuKey Laboratory of Carbohydrate Chemistry and Biotechnology, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.
Jinling ZhanKey Laboratory of Carbohydrate Chemistry and Biotechnology, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.
Zhonghu BaiKey Laboratory of Carbohydrate Chemistry and Biotechnology, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.
Jiangnan University · CNRensselaer Polytechnic Institute · US

Funding

national first-class discipline program of Light Industry Technology and Engineering LITE2018-24National Natural Science Foundation of China 21908077National Natural Science Foundation of China 31570034Six Talent Peaks Project in Jiangsu Province SWYY-180the 111 Project 111-2-06
6 · The paper itself

Abstract

Pichia pastoris is able to metabolize methanol via a specific MUT (methanol utilization) pathway. Based on the powerful AOX1 (Alcohol Oxidase 1) promoter, the P. pastoris expression system has become one of the most widely used eukaryotic expression systems. The molecular mechanisms of methanol metabolic regulation remain unclearly understood, so it is important to identify and develop new transcriptional regulators. Our previous studies suggested that the expression of SUT2 could be induced by methanol but is repressed by glycerol, which indicates that SUT2 may be involved in methanol metabolism through an unknown mechanism. SUT2 encodes a putative transcription factor-like protein harboring a Gal4-like Zn

Indexed as

Aldehyde OxidaseBinding SitesFungal ProteinsGene Expression Regulation, FungalMethanolPichiaPromoter Regions, GeneticSequence DeletionTranscription FactorsTranscription Initiation SiteAldehyde OxidaseFungal ProteinsMethanolTranscription FactorsForward-loop cassetteMethanol utilization (MUT) pathwayPichia pastorisPromoterSUT2Transcriptional regulation

Identifiers

PMID32566991
OpenAlexW3036514270

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.