ArticleBone & joint research2020
Preliminary evaluation of a rapid lateral flow calprotectin test for the diagnosis of prosthetic joint infection.
Article in Bone & joint research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 5 of them syntheses that pooled it.
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Who cites it
15 citing papers in PubMed, 5 syntheses or guidelines pooled it, 33 citations in OpenAlex.
- Is synovial calprotectin lateral flow test a reliable intraoperative biomarker for periprosthetic joint infection? A systematic review and meta-analysis.Archives of orthopaedic and trauma surgery · 2026Pooled it
- Synovial calprotectin in prosthetic joint infection. A systematic review and meta-analysis of the literature.Archives of orthopaedic and trauma surgery · 2024Pooled it
- Reliable Diagnostic Tests and Thresholds for Preoperative Diagnosis of Non-Inflammatory Arthritis Periprosthetic Joint Infection: A Meta-analysis and Systematic Review.Orthopaedic surgery · 2022Pooled it
- Diagnostic accuracy of calprotectin in periprosthetic joint infection: a diagnostic meta-analysis.Journal of orthopaedic surgery and research · 2022Pooled it
- Synovial calprotectin for the diagnosis of periprosthetic joint infection: a diagnostic meta-analysis.Journal of orthopaedic surgery and research · 2022Pooled it
- Synovial Calprotectin in Suspected Periprosthetic Joint Infection After Total Knee Arthroplasty: Diagnostic Accuracy and Exploratory Adjunctive Value to Preoperative ICM Classification.Antibiotics (Basel, Switzerland) · 2026Article
- Review
- Comparison of synovial calprotectin and alpha-defensin for the diagnosis of persistent periprosthetic joint infections at second stage of a two-stage revision arthroplasty.Archives of orthopaedic and trauma surgery · 2025Article
- Synovial fluid calprotectin quantitative rapid diagnostic biomarker for risk stratification of periprosthetic joint infections.European journal of orthopaedic surgery & traumatology : orthopedie traumatologie · 2025Article
- Synovial fluid alpha-defensins in Lyme arthritis-a useful marker.Folia microbiologica · 2024Article
- Profiling the Immune Response to Periprosthetic Joint Infection and Non-Infectious Arthroplasty Failure.Antibiotics (Basel, Switzerland) · 2023Review
- Utility of synovial calprotectin lateral flow test to exclude chronic prosthetic joint infection in periprosthetic fractures: a prospective cohort study.Scientific reports · 2022Article
- Synovial calprotectin is a reliable biomarker for periprosthetic joint infections in acute-phase inflammation - a prospective cohort study.International orthopaedics · 2022Article
- Application ofBone & joint research · 2022Article
- Synovial Calprotectin for Diagnosing Periprosthetic Joint Infection in Loose Hip and Knee Arthroplasties: A Prospective Cohort Study.In vivo (Athens, Greece)Article
Corrections and comments
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Authors and funding
12 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimsThis pilot study tested the performance of a rapid assay for diagnosing prosthetic joint infection (PJI), which measures synovial fluid calprotectin from total hip and knee revision patients.
methodsA convenience series of 69 synovial fluid samples from revision patients at the Norfolk and Norwich University Hospital were collected intraoperatively (52 hips, 17 knees) and frozen. Synovial fluid calprotectin was measured retrospectively using a new commercially available lateral flow assay for PJI diagnosis (Lyfstone AS) and compared to International Consensus Meeting (ICM) 2018 criteria and clinical case review (ICM-CR) gold standards.
resultsAccording to ICM, 24 patients were defined as PJI positive and the remaining 45 were negative. The overall accuracy of the lateral flow test compared to ICM was 75.36% (52/69, 95% CI 63.51% to 84.95%), sensitivity and specificity were 75.00% (18/24, 95% CI 53.29% to 90.23%) and 75.56% (34/45, 95% CI 60.46% to 87.12%), respectively, positive predictive value (PPV) was 62.07% (18/29, 95% CI 48.23% to 74.19%) and negative predictive value (NPV) was 85.00% (34/40, 95% CI 73.54% to 92.04%), and area under the receiver operating characteristic (ROC) curve (AUC) was 0.78 (95% CI 0.66 to 0.87). Patient data from discordant cases were reviewed by the clinical team to develop the ICM-CR gold standard. The lateral flow test performance improved significantly when compared to ICM-CR, with accuracy increasing to 82.61% (57/69, 95% CI 71.59% to 90.68%), sensitivity increasing to 94.74% (18/19, 95% CI 73.97% to 99.87%), NPV increasing to 97.50% (39/40, 95% CI 85.20% to 99.62%), and AUC increasing to 0.91 (95% CI 0.81 to 0.96). Test performance was better in knees (100.00% accurate (17/17, 95% CI 80.49% to 100.00%)) compared to hips (76.92% accurate (40/52, 95% CI 63.16% to 87.47%)).
conclusionThis study demonstrates that the calprotectin lateral flow assay could be an effective diagnostic test for PJI, however additional prospective studies testing fresh samples are required.
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