Evidence map›Paper›PMID 32564264›Full record

ArticlePathology oncology research : POR2020

Label-Free Semiquantitative Liquid Chromatography-Tandem Mass Spectrometry Proteomics Analysis of Laryngeal/Hypopharyngeal Squamous Cell Carcinoma on Formalin-Fixed, Paraffin-Embedded Tissue Samples - a Pilot Study.

Andras Burian, Laszlo Lujber, Imre Gerlinger, Tamas Jarai, Eva Orosz, Lilla Turiak, Andras Acs, Zoltan Hegedus, Aniko Konigne Peter, Tamas Tornoczki and 2 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Pathology oncology research : POR, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.5field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 1 country.

Andras BurianClinical Centre, Department of Otorhinolaryngology and Head and Neck Surgery, University of Pecs, Munkacsy M Str 2, Pecs, H-7621, Hungary.
Laszlo LujberClinical Centre, Department of Otorhinolaryngology and Head and Neck Surgery, University of Pecs, Munkacsy M Str 2, Pecs, H-7621, Hungary.
Imre GerlingerClinical Centre, Department of Otorhinolaryngology and Head and Neck Surgery, University of Pecs, Munkacsy M Str 2, Pecs, H-7621, Hungary.
Tamas JaraiTolna County Balassa Janos Hospital, Beri Balogh Adam Str 5-7, Szekszard, H-7100, Hungary.
Eva OroszClinical Centre, Department of Otorhinolaryngology and Head and Neck Surgery, University of Pecs, Munkacsy M Str 2, Pecs, H-7621, Hungary.
Lilla TuriakMS Proteomics Research Group, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Magyar tudosok Blvd 2, Budapest, H-1117, Hungary.
Andras AcsMS Proteomics Research Group, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Magyar tudosok Blvd 2, Budapest, H-1117, Hungary.
Zoltan HegedusBiological Research Centre of the Hungarian Academy of Sciences, Institute of Biophysics, Temesvari Blvd 62, Szeged, H-6726, Hungary.
Aniko Konigne PeterMedical School, Institute of Bioanalysis, University of Pecs, Honved Str 1, Pecs, H-7624, Hungary.
Tamas TornoczkiMedical School, Institute of Pathology, University of Pecs, Szigeti Str 12, Pecs, H-7624, Hungary.
Katalin GombosClinical Centre, Department of Laboratory Medicine, University of Pecs, Ifjusag Str 13, Pecs, H-7624, Hungary.
Laszlo MarkMedical School, Institute of Biochemistry and Medical Chemistry, University of Pecs, Szigeti Str 12, Pecs, H-7624, Hungary. laszlo.mark@aok.pte.hu.
University of Pecs · HUHungarian Academy of Sciences · HUHUN-REN Research Centre for Natural Sciences · HUSzent János Kórház · HU

Funding

Nemzeti Kutatási Fejlesztési és Innovációs Hivatal GINOP-2.3.2-15Nemzeti Kutatási Fejlesztési és Innovációs Hivatal NKFIH PD-121187
6 · The paper itself

Abstract

Squamous cell carcinoma (SCC) of the head and neck region is the sixth most frequent malignancy with high mortality rate. Due to its poor prognosis it is considered a growing public health problem worldwide inspite of existing treatment modalities. Thus, early diagnosis of new diseases and recurrences is emerging on one hand, but on the other hand troublesome in the lack of reliable tumor markers in this field. The rapid development of proteomics has opened new perspectives in tumor marker discovery. Liquid chromatography/mass spectrometry (LC/MS) as the gold standard in proteomics enables the semi-quantitative analysis of proteins within various tissues. Abundance differences between tumor and normal tissue also can be interpreted as tumor specific changes. The aim of this study was to identify potential tumor markers of laryngeal/hypopharyngeal SCC by revealing abundance changes between cancerous and the surrounding phenotypically healthy tissue. After separating the phenotypically cancerous and healthy parts of formalin-fixed paraffin-embedded tissues, each sample underwent protein recovery process and tryptic digestion for label-free semi-quantitative LC/MS analysis. Eight proteins showed significantly higher abundance in tumor including tenascin, transmembrane emp24 domain-containing protein 2, cytoplasmic dynein light chain 1, coactosin-like protein, small proline-rich protein 2D, nucleolin, U5 small nuclear RNP 200-kDa helicase and fatty aldehyde dehydrogenase. Desmoglein-1 and keratin type I cytoskeletal 9 were down-regulated in tumor. Using Ingenuity Pathway Analysis we mapped the signaling pathways these proteins play role in regarding other tumors. Based on these findings these proteins may serve as promising biomarkers in the fight against laryngeal/hypopharyngeal SCCs.

Indexed as

AgedBiomarkers, TumorCarcinoma, Squamous CellChromatography, LiquidFemaleFollow-Up StudiesFormaldehydeHumansHypopharyngeal NeoplasmsLaryngeal NeoplasmsMaleMiddle AgedParaffin EmbeddingPilot ProjectsPrognosisProteomeBiomarkers, TumorFormaldehydeProteomeBiomarker discoveryLaryngeal cancerLC/MSProteomicsSquamous cell carcinoma

Identifiers

PMID32564264
PMCPMC7471140
OpenAlexW3036427329

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.