Evidence map›Paper›PMID 32562942›Full record

ArticleBiomaterials2020

Engineering 3D skeletal muscle primed for neuromuscular regeneration following volumetric muscle loss.

Jordana Gilbert-Honick, Shama R Iyer, Sarah M Somers, Hannah Takasuka, Richard M Lovering, Kathryn R Wagner, Hai-Quan Mao, Warren L Grayson

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomaterials, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.

0numbers the graph read from it
0cells of the map it votes in
39citing papers in PubMed
–field-weighted citation impact, top 100% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

39 citing papers in PubMed, 0 citations in OpenAlex.

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  20. 3D Printing of Self-Assembling Nanofibrous Multidomain Peptide Hydrogels.Advanced materials (Deerfield Beach, Fla.) · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Jordana Gilbert-HonickTranslational Tissue Engineering Center, School of Medicine, Johns Hopkins University, Baltimore, MD 21231, USA; Department of Biomedical Engineering, School of Medicine, Johns Hopkins University, Baltimore, MD 21205, USA.
Shama R IyerDepartment of Orthopedic Surgery, School of Medicine, University of Maryland, Baltimore, MD 21201, USA.
Sarah M SomersTranslational Tissue Engineering Center, School of Medicine, Johns Hopkins University, Baltimore, MD 21231, USA; Department of Biomedical Engineering, School of Medicine, Johns Hopkins University, Baltimore, MD 21205, USA.
Hannah TakasukaTranslational Tissue Engineering Center, School of Medicine, Johns Hopkins University, Baltimore, MD 21231, USA; Department of Biomedical Engineering, School of Medicine, Johns Hopkins University, Baltimore, MD 21205, USA.
Richard M LoveringDepartment of Orthopedic Surgery, School of Medicine, University of Maryland, Baltimore, MD 21201, USA.
Kathryn R WagnerThe Hugo W. Moser Research Institute, Kennedy Krieger Institute, Baltimore, MD 21205, USA; Graduate Program in Cellular and Molecular Medicine, School of Medicine, Johns Hopkins University, Baltimore, MD 21205, USA; Departments of Neurology and Neuroscience, School of Medicine, Johns Hopkins University, Baltimore, MD 21205, USA.
Hai-Quan MaoTranslational Tissue Engineering Center, School of Medicine, Johns Hopkins University, Baltimore, MD 21231, USA; Department of Biomedical Engineering, School of Medicine, Johns Hopkins University, Baltimore, MD 21205, USA; Department of Materials Science & Engineering, Whiting School of Engineering, Johns Hopkins University, Baltimore, MD 21218, USA; Institute for NanoBioTechnology (INBT), Johns Hopkins University School of Engineering, Baltimore, MD 21218, USA.
Warren L GraysonTranslational Tissue Engineering Center, School of Medicine, Johns Hopkins University, Baltimore, MD 21231, USA; Department of Biomedical Engineering, School of Medicine, Johns Hopkins University, Baltimore, MD 21205, USA; Department of Materials Science & Engineering, Whiting School of Engineering, Johns Hopkins University, Baltimore, MD 21218, USA; Institute for NanoBioTechnology (INBT), Johns Hopkins University School of Engineering, Baltimore, MD 21218, USA. Electronic address: wgrayson@jhmi.edu.
Johns Hopkins University · USUniversity of Maryland, Baltimore · USKennedy Krieger Institute · US

Funding

Wilmer Core Grant for Vision ResearchP30EY001765 · NEI · JOHNS HOPKINS UNIVERSITY · PI Ji Yi · 1985 to 2026
$22.1M
Regenerating Vascularized and Innervated Skeletal Muscle to Treat VML DefectsR01AR077581 · NIAMS · JOHNS HOPKINS UNIVERSITY · PI GRAYSON, WARREN L, LEE, GABSANG · 2020 to 2024
$2.2M
Altered nucleus-cytoskeleton coupling in dystrophic muscleK01AR074048 · NIAMS · UNIVERSITY OF MARYLAND BALTIMORE · PI IYER, SHAMA RAJAN · 2019 to 2023
$420k
Engineering Vascularized Skeletal Muscle Tissues with Adipose-derived Stem CellsF31AR071759 · NIAMS · JOHNS HOPKINS UNIVERSITY · PI GILBERT-HONICK, JORDANA · 2018 to 2019
$89k
NEI NIH HHS P30 EY001765NIAMS NIH HHS F31 AR071759NIAMS NIH HHS K01 AR074048NIAMS NIH HHS R01 AR077581
6 · The paper itself

Abstract

Volumetric muscle loss (VML) overwhelms the native regenerative capabilities of skeletal muscle and has few effective treatments to regain lost muscle mass and function. Tissue engineered muscle constructs designed to promote neuromuscular regeneration are a promising therapeutic avenue. To date, there has been no engineered muscle construct for VML treatment that has incorporated a pharmacologic agent to promote neuromuscular regeneration. Here, we have modified electrospun fibrin microfiber bundles, which have demonstrated muscle regenerative potential, with the heparan sulfate proteoglycan, agrin, to stimulate innervation post-VML. Myoblasts cultured on microfiber bundles with either soluble or chemically tethered agrin demonstrated statistically significant increased clustering of acetylcholine receptors (AChRs) with soluble agrin displaying AChR clusters throughout the myofiber bundles, and tethered agrin displaying AChR clusters only at 10 μm from the substrate surface. Following implantation into murine VML defects for 4 weeks, constructs pre-treated with soluble or tethered agrin resulted in statistically significant increased neuromuscular junctions, regenerating myofibers, vascular infiltration, neural infiltration, and nuclear yes-associated protein (YAP) expression within the defect site compared to the control without agrin. The agrin-tethered microfiber bundles provided sustained agrin signaling within the regenerating site during the 4-week post-implantation periods and further augmented the density of regenerating myofibers in regenerated tissue with statistical significance compared to constructs with soluble agrin. These data demonstrate the neuromuscular regenerative potential of engineered muscle constructs pre-treated to induce AChR clustering with locally delivered agrin at the site of VML regeneration.

Indexed as

AgrinMuscle, SkeletalAnimalsMiceMuscle Fibers, SkeletalNeuromuscular JunctionReceptors, CholinergicRegenerationAgrinReceptors, CholinergicAgrinElectrospun hydrogelsNeuromuscular junctionTissue engineered skeletal muscleVolumetric muscle loss

Identifiers

PMID32562942
PMCPMC11192434
OpenAlexW2969581495

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.