ReviewExperimental cell research2020
Identification of immune and non-immune cells in regenerating axolotl limbs by single-cell sequencing.
Review in Experimental cell research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
25 citing papers in PubMed, 43 citations in OpenAlex.
- Article
- Allogeneic skin transplantation induces transient fibrosis that undergoes spontaneous regression in newts.Inflammation and regeneration · 2026Article
- Are axolotls resistant to cancer? Possible explanations.Molecular biology reports · 2026Review
- Comparative multi-omic analysis reveals conserved and derived mechanisms of fin and limb regeneration.Nature communications · 2026Article
- Ependymoglial cells are critical for cortex regeneration in axolotls.Nature communications · 2026Article
- Article
- Single-cell RNA sequencing of the holothurian regenerating intestine reveals the pluripotency of the coelomic epithelium.bioRxiv : the preprint server for biology · 2024Article
- Regeneration in Mice of Injured Skin, Heart, and Spinal Cord by α-Gal Nanoparticles Recapitulates Regeneration in Amphibians.Nanomaterials (Basel, Switzerland) · 2024Review
- Cellular and Molecular Triggers of Retinal Regeneration in Amphibians.Life (Basel, Switzerland) · 2023Review
- Evaluation of genetic demultiplexing of single-cell sequencing data from model species.Life science alliance · 2023Article
- Structural and functional analysis of the newt lymphatic system.Scientific reports · 2023Article
- Now that We Got There, What Next?Methods in molecular biology (Clifton, N.J.) · 2023Article
- Article
- Rebuilding limbs, one cell at a time.Developmental dynamics : an official publication of the American Association of Anatomists · 2022Article
- The amazing and anomalous axolotls as scientific models.Developmental dynamics : an official publication of the American Association of Anatomists · 2022Article
- Article
- The Role of MSCs and Cell Fusion in Tissue Regeneration.International journal of molecular sciences · 2021Review
- Appendage regeneration is context dependent at the cellular level.Open biology · 2021Article
- Immunity in salamander regeneration: Where are we standing and where are we headed?Developmental dynamics : an official publication of the American Association of Anatomists · 2021Review
- Chemical genetics of regeneration: Contrasting temporal effects of CoClDevelopmental dynamics : an official publication of the American Association of Anatomists · 2021Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Immune cells are known to be critical for successful limb regeneration in the axolotl (Ambystoma mexicanum), but many details regarding their identity, behavior, and function are yet to be resolved. We isolated peripheral leukocytes from the blood of adult axolotls and then created two samples for single-cell sequencing: 1) peripheral leukocytes (N = 7889) and 2) peripheral leukocytes with presumptive macrophages from the intraperitoneal cavity (N = 4998). Using k-means clustering, we identified 6 cell populations from each sample that presented gene expression patterns indicative of erythrocyte, thrombocyte, neutrophil, B-cell, T-cell, and myeloid cell populations. A seventh, presumptive macrophage cell population was identified uniquely from sample 2. We then isolated cells from amputated axolotl limbs at 1 and 6 days post-amputation (DPA) and performed single cell sequencing (N = 8272 and 9906 cells respectively) to identify immune and non-immune cell populations. Using k-means clustering, we identified 8 cell populations overall, with the majority of cells expressing erythrocyte-specific genes. Even though erythrocytes predominated, we used an unbiased approach to identify infiltrating neutrophil, macrophage, and lymphocyte populations at both time points. Additionally, populations expressing genes for epidermal cells, fibroblast-like cells, and endothelial cells were also identified. Consistent with results from previous experimental studies, neutrophils were more abundant at 1 DPA than 6 DPA, while macrophages and non-immune cells exhibited inverse abundance patterns. Of note, we identified a small population of fibroblast-like cells at 1 DPA that was represented by considerably more cells at 6 DPA. We hypothesize that these are early progenitor cells that give rise to the blastema. The enriched gene sets from our work will aid future single-cell investigations of immune cell diversity and function during axolotl limb regeneration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.